Differential Sox10 genomic occupancy in myelinating glia

Camila Lopez-Anido, Guannan Sun, Matthias Koenning, Rajini Srinivasan, Holly A. Hung, Ben Emery, Sunduz Keles, John Svaren

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Myelin is formed by specialized myelinating glia: oligodendrocytes and Schwann cells in the central and peripheral nervous systems, respectively. While there are distinct developmental aspects and regulatory pathways in these two cell types, myelination in both systems requires the transcriptional activator Sox10. Sox10 interacts with cell type-specific transcription factors at some loci to induce myelin gene expression, but it is largely unknown how Sox10 transcriptional networks globally compare between oligodendrocytes and Schwann cells. We used in vivo ChIP-Seq analysis of spinal cord and peripheral nerve (sciatic nerve) to identify unique and shared Sox10 binding sites and assess their correlation with active enhancers and transcriptional profiles in oligodendrocytes and Schwann cells. Sox10 binding sites overlap with active enhancers and critical cell type-specific regulators of myelination, such as Olig2 and Myrf in oligodendrocytes, and Egr2/Krox20 in Schwann cells. Sox10 sites also associate with genes critical for myelination in both oligodendrocytes and Schwann cells and are found within super-enhancers previously defined in brain. In Schwann cells, Sox10 sites contain binding motifs of putative partners in the Sp/Klf, Tead, and nuclear receptor protein families. Specifically, siRNA analysis of nuclear receptors Nr2f1 and Nr2f2 revealed downregulation of myelin genes Mbp and Ndrg1 in primary Schwann cells. Our analysis highlights different mechanisms that establish cell type-specific genomic occupancy of Sox10, which reflects the unique characteristics of oligodendrocyte and Schwann cell differentiation.

Original languageEnglish (US)
Pages (from-to)1897-1914
Number of pages18
JournalGLIA
Volume63
Issue number11
DOIs
StatePublished - Nov 1 2015
Externally publishedYes

Fingerprint

Schwann Cells
Neuroglia
Oligodendroglia
Myelin Sheath
Binding Sites
Cytoplasmic and Nuclear Receptors
Spinal Nerves
Gene Regulatory Networks
Peripheral Nervous System
Sciatic Nerve
Nuclear Proteins
Peripheral Nerves
Small Interfering RNA
Genes
Cell Differentiation
Spinal Cord
Transcription Factors
Down-Regulation
Central Nervous System
Gene Expression

Keywords

  • Chromatin
  • Enhancer
  • Myelin
  • Oligodendrocyte
  • Schwann
  • Transcription

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology

Cite this

Lopez-Anido, C., Sun, G., Koenning, M., Srinivasan, R., Hung, H. A., Emery, B., ... Svaren, J. (2015). Differential Sox10 genomic occupancy in myelinating glia. GLIA, 63(11), 1897-1914. https://doi.org/10.1002/glia.22855

Differential Sox10 genomic occupancy in myelinating glia. / Lopez-Anido, Camila; Sun, Guannan; Koenning, Matthias; Srinivasan, Rajini; Hung, Holly A.; Emery, Ben; Keles, Sunduz; Svaren, John.

In: GLIA, Vol. 63, No. 11, 01.11.2015, p. 1897-1914.

Research output: Contribution to journalArticle

Lopez-Anido, C, Sun, G, Koenning, M, Srinivasan, R, Hung, HA, Emery, B, Keles, S & Svaren, J 2015, 'Differential Sox10 genomic occupancy in myelinating glia', GLIA, vol. 63, no. 11, pp. 1897-1914. https://doi.org/10.1002/glia.22855
Lopez-Anido C, Sun G, Koenning M, Srinivasan R, Hung HA, Emery B et al. Differential Sox10 genomic occupancy in myelinating glia. GLIA. 2015 Nov 1;63(11):1897-1914. https://doi.org/10.1002/glia.22855
Lopez-Anido, Camila ; Sun, Guannan ; Koenning, Matthias ; Srinivasan, Rajini ; Hung, Holly A. ; Emery, Ben ; Keles, Sunduz ; Svaren, John. / Differential Sox10 genomic occupancy in myelinating glia. In: GLIA. 2015 ; Vol. 63, No. 11. pp. 1897-1914.
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