Differential response of cultured mouse mammary cells of varying tumorigenicity to cytochalasin B

M. R. Steiner, B. Altenburg, Carolyn (Sue) Richards, J. P. Dudley, D. Medina, J. S. Butel

Research output: Contribution to journalArticle

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Abstract

Cultured BALB/c mouse mammary gland epithelial cells of varying oncogenic potential in vivo have been examined for their ability to multinucleate in the presence of cytochalasin B (CB). Highly tumorigenic cell lines derived from mammary tumors with hormonal, viral, or chemical carcinogen etiologies were extensively multinucleated when cultured in CB-supplemented medium. Normal mammary gland cells from either pregnant or lactating animals were predominantly mono- or binucleate under comparable conditions. At low passage levels after cloning, cell lines derived from a chemical carcinogen-induced mammary tumor were weakly oncogenic and remained largely mono-, or binucleated when cultured in CB-supplemented medium. At higher cell passage levels, both the ability to produce tumors in vivo and the degree of multinucleation in CB-supplemented medium increased dramatically with the clonal cell lines. Thus, the response of cultured mouse mammary gland epithelial cells to CB in vitro may be useful as a marker of the oncogenic potential of such cells.

Original languageEnglish (US)
Pages (from-to)2719-2721
Number of pages3
JournalCancer Research
Volume38
Issue number9
StatePublished - 1978
Externally publishedYes

Fingerprint

Cytochalasin B
Breast
Human Mammary Glands
Cell Line
Carcinogens
Epithelial Cells
Breast Neoplasms
Organism Cloning
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Steiner, M. R., Altenburg, B., Richards, C. S., Dudley, J. P., Medina, D., & Butel, J. S. (1978). Differential response of cultured mouse mammary cells of varying tumorigenicity to cytochalasin B. Cancer Research, 38(9), 2719-2721.

Differential response of cultured mouse mammary cells of varying tumorigenicity to cytochalasin B. / Steiner, M. R.; Altenburg, B.; Richards, Carolyn (Sue); Dudley, J. P.; Medina, D.; Butel, J. S.

In: Cancer Research, Vol. 38, No. 9, 1978, p. 2719-2721.

Research output: Contribution to journalArticle

Steiner, MR, Altenburg, B, Richards, CS, Dudley, JP, Medina, D & Butel, JS 1978, 'Differential response of cultured mouse mammary cells of varying tumorigenicity to cytochalasin B', Cancer Research, vol. 38, no. 9, pp. 2719-2721.
Steiner, M. R. ; Altenburg, B. ; Richards, Carolyn (Sue) ; Dudley, J. P. ; Medina, D. ; Butel, J. S. / Differential response of cultured mouse mammary cells of varying tumorigenicity to cytochalasin B. In: Cancer Research. 1978 ; Vol. 38, No. 9. pp. 2719-2721.
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