Differential Response of Cultured Mouse Mammary Cells of Varying Tumorigenicity to Cytochalasin B

Marion R. Steiner, Betty Altenburg, Carolyn S. Richards, Jaquelin P. Dudley, Daniel Medina, Janet S. Butel

Research output: Contribution to journalArticlepeer-review

23 Scopus citations

Abstract

Cultured BALB/c mouse mammary gland epithelial cells of varying oncogenic potential in vivo have been examined for their ability to multinucleate in the presence of cytochalasin B (CB). Highly tumorigenic cell lines derived from mammary tumors with hormonal, viral, or chemical carcinogen etiologies were extensively multinucleated when cultured in CB-supplemented medium. Normal mammary gland cells from either pregnant or lactating animals were predominantly mono-or binucleate under comparable conditions. At low passage levels after cloning, cell lines derived from a chemical carcinogen-induced mammary tumor were weakly oncogenic and remained largely mono-, or binucleated when cultured in CB-supple-mented medium. At higher cell passage levels, both the ability to produce tumors in vivo and the degree of multinucleation in CB-supplemented medium increased dramatically with the clonal cell lines. Thus, the response of cultured mouse “mammary gland epithelial cells to CB in vitro may be useful as a marker of the oncogenic potential of such cells.

Original languageEnglish (US)
Pages (from-to)2719-2721
Number of pages3
JournalCancer Research
Volume38
Issue number9
StatePublished - Sep 1978
Externally publishedYes

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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