Abstract
Nonshivering thermogenesis in brown adipose tissue (BAT) provides heat through activation of a mitochondrial uncoupling protein (UCP1), which causes futile electron transport cycles without the production of ATP. Recent discovery of two molecular homologues, UCP2, expressed in multiple tissues, and UCP3, expressed in muscle, has resulted in investigation of their roles in thermoregulatory physiology and energy balance. To determine the expression pattern of Ucp homologues in hibernating mammals, we compared relative mRNA levels of Ucp1, -2, and -3 in BAT, white adipose tissue (WAT), and skeletal muscle of arctic ground squirrels (Spermophilus parryii) hibernating at different ambient and body temperatures, with levels determined in tissues from ground squirrels not in hibernation. Here we report significant increases in mRNA levels for Ucp2 in WAT (1.6-fold) and Ucp3 in skeletal muscle (3-fold) during hibernation. These results indicate the potential for a role of UCP2 and UCP3 in thermal homeostasis during hibernation and indicate that parallel mechanisms and multiple tissues could be important for nonshivering thermoregulation in mammals.
| Original language | English (US) |
|---|---|
| Journal | American Journal of Physiology - Regulatory Integrative and Comparative Physiology |
| Volume | 275 |
| Issue number | 4 44-4 |
| State | Published - Oct 1 1998 |
| Externally published | Yes |
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Keywords
- Nonshivering thermogenesis
- Spermophilus
- Uncoupling protein 1
- Uncoupling protein 2
- Uncoupling protein 3
ASJC Scopus subject areas
- Physiology
- Physiology (medical)
Cite this
Differential regulation of uncoupling protein gene homologues in multiple tissues of hibernating ground squirrels. / Boyer, Bert; Barnes, Brian M.; Lowell, Bradford B.; Grujic, Danica.
In: American Journal of Physiology - Regulatory Integrative and Comparative Physiology, Vol. 275, No. 4 44-4, 01.10.1998.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential regulation of uncoupling protein gene homologues in multiple tissues of hibernating ground squirrels
AU - Boyer, Bert
AU - Barnes, Brian M.
AU - Lowell, Bradford B.
AU - Grujic, Danica
PY - 1998/10/1
Y1 - 1998/10/1
N2 - Nonshivering thermogenesis in brown adipose tissue (BAT) provides heat through activation of a mitochondrial uncoupling protein (UCP1), which causes futile electron transport cycles without the production of ATP. Recent discovery of two molecular homologues, UCP2, expressed in multiple tissues, and UCP3, expressed in muscle, has resulted in investigation of their roles in thermoregulatory physiology and energy balance. To determine the expression pattern of Ucp homologues in hibernating mammals, we compared relative mRNA levels of Ucp1, -2, and -3 in BAT, white adipose tissue (WAT), and skeletal muscle of arctic ground squirrels (Spermophilus parryii) hibernating at different ambient and body temperatures, with levels determined in tissues from ground squirrels not in hibernation. Here we report significant increases in mRNA levels for Ucp2 in WAT (1.6-fold) and Ucp3 in skeletal muscle (3-fold) during hibernation. These results indicate the potential for a role of UCP2 and UCP3 in thermal homeostasis during hibernation and indicate that parallel mechanisms and multiple tissues could be important for nonshivering thermoregulation in mammals.
AB - Nonshivering thermogenesis in brown adipose tissue (BAT) provides heat through activation of a mitochondrial uncoupling protein (UCP1), which causes futile electron transport cycles without the production of ATP. Recent discovery of two molecular homologues, UCP2, expressed in multiple tissues, and UCP3, expressed in muscle, has resulted in investigation of their roles in thermoregulatory physiology and energy balance. To determine the expression pattern of Ucp homologues in hibernating mammals, we compared relative mRNA levels of Ucp1, -2, and -3 in BAT, white adipose tissue (WAT), and skeletal muscle of arctic ground squirrels (Spermophilus parryii) hibernating at different ambient and body temperatures, with levels determined in tissues from ground squirrels not in hibernation. Here we report significant increases in mRNA levels for Ucp2 in WAT (1.6-fold) and Ucp3 in skeletal muscle (3-fold) during hibernation. These results indicate the potential for a role of UCP2 and UCP3 in thermal homeostasis during hibernation and indicate that parallel mechanisms and multiple tissues could be important for nonshivering thermoregulation in mammals.
KW - Nonshivering thermogenesis
KW - Spermophilus
KW - Uncoupling protein 1
KW - Uncoupling protein 2
KW - Uncoupling protein 3
UR - http://www.scopus.com/inward/record.url?scp=0031768872&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031768872&partnerID=8YFLogxK
M3 - Article
C2 - 9756555
AN - SCOPUS:0031768872
VL - 275
JO - American Journal of Physiology
JF - American Journal of Physiology
SN - 0363-6119
IS - 4 44-4
ER -