Differential regulation of uncoupling protein gene homologues in multiple tissues of hibernating ground squirrels

Bert B. Boyer, Brian M. Barnes, Bradford B. Lowell, Danica Grujic

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

Nonshivering thermogenesis in brown adipose tissue (BAT) provides heat through activation of a mitochondrial uncoupling protein (UCP1), which causes futile electron transport cycles without the production of ATP. Recent discovery of two molecular homologues, UCP2, expressed in multiple tissues, and UCP3, expressed in muscle, has resulted in investigation of their roles in thermoregulatory physiology and energy balance. To determine the expression pattern of Ucp homologues in hibernating mammals, we compared relative mRNA levels of Ucp1, -2, and -3 in BAT, white adipose tissue (WAT), and skeletal muscle of arctic ground squirrels (Spermophilus parryii) hibernating at different ambient and body temperatures, with levels determined in tissues from ground squirrels not in hibernation. Here we report significant increases in mRNA levels for Ucp2 in WAT (1.6-fold) and Ucp3 in skeletal muscle (3-fold) during hibernation. These results indicate the potential for a role of UCP2 and UCP3 in thermal homeostasis during hibernation and indicate that parallel mechanisms and multiple tissues could be important for nonshivering thermoregulation in mammals.

Original languageEnglish (US)
Pages (from-to)R1232-R1238
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume275
Issue number4 44-4
StatePublished - Oct 1 1998

Keywords

  • Nonshivering thermogenesis
  • Spermophilus
  • Uncoupling protein 1
  • Uncoupling protein 2
  • Uncoupling protein 3

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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