Differential regulation of mannose 6-phosphate receptors and their ligands during the myogenic development of C2 cells

Gyorgyi Szebenyi, Peter Rotwein

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The mammalian insulin-like growth factor II/cation-independent mannose 6-phosphate receptor (IGF-II/CIMPR) mediates both targeting and endocytosis of mannose 6-phosphate-containing proteins and binds insulin-like growth factor II (IGF-II). The cation-dependent mannose 6-phosphate receptor (CDMPR) lacks an IGF-II-binding site and participates only in the intracellular trafficking of lysosomal enzymes. During terminal differentiation of the myogenic C2 cell line, there is an increase in cell surface expression of the IGF-II/CIMPR in parallel with a rise in secretion of IGF-II (Tollefsen, S. E., Sadow, J. L., and Rotwein, P. (1989) Proc. Natl. Acad. Sci. U. S. A. 86, 1543-1547). In this study we show that IGF-II/CIMPR mRNA increases by more than 10-fold during the initial 48 h of C2 muscle differentiation with kinetics similar to the rise in IGF-II mRNA. Comparable levels of both mRNAs are expressed in C2 myotubes and in primary cultures of fetal muscle. By contrast, no change is observed in CDMPR transcript abundance during differentiation, and only a small, transient increase is seen in the enzymatic activities and mRNA levels of several lysosomal enzymes. The differential regulation of the two mannose 6-phosphate receptors during muscle differentiation suggests that they may serve distinct functions in development.

Original languageEnglish (US)
Pages (from-to)5534-5539
Number of pages6
JournalJournal of Biological Chemistry
Volume266
Issue number9
StatePublished - 1991
Externally publishedYes

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IGF Type 2 Receptor
Insulin-Like Growth Factor II
Ligands
Muscle
Cations
Messenger RNA
Muscles
Skeletal Muscle Fibers
Enzymes
Endocytosis
Binding Sites
Cells
Cell Line
Kinetics

ASJC Scopus subject areas

  • Biochemistry

Cite this

Differential regulation of mannose 6-phosphate receptors and their ligands during the myogenic development of C2 cells. / Szebenyi, Gyorgyi; Rotwein, Peter.

In: Journal of Biological Chemistry, Vol. 266, No. 9, 1991, p. 5534-5539.

Research output: Contribution to journalArticle

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