TY - JOUR
T1 - Differential regulation of genes encoding synaptic proteins by members of the Brn-3 subfamily of POU transcription factors
AU - Morris, Peter J.
AU - Lakin, Nick D.
AU - Dawson, Sally J.
AU - Ryabinin, Andrey E.
AU - Kilimann, Manfred W.
AU - Wilson, Michael C.
AU - Latchman, David S.
N1 - Funding Information:
We thank Dr. T. Moroy for the Brn-3 expression vectors. This work was supported by the Medical Research Council and WellBeing (D.S.L.) and by NIH Grant MH48989 (M.C.W.) and by the Deutsche Forschungsgemeinschaft (M.W.K).
PY - 1996/12/31
Y1 - 1996/12/31
N2 - The three members of the Brn-3 subfamily of POU transcription factors have distinct effects on target gene expression. We show that the promoter of the gene encoding the presynaptic nerve terminal protein SNAP-25 resembles previously characterised target genes in being activated by Brn-3a and Brn-3c, but being repressed by Brn-3b. Unlike other target genes, however, the SNAP-25 promoter can be activated by either the N- or C-terminal activation domains of Brn-3a. In contrast to the SNAP-25 gene, the gene encoding the synaptic vesicle protein synapsin I is activated by all the Bm-3 factors, the first gene for which this activation pattern has been reported. Interestingly, however, similar activation by all three Brn-3 factors can be observed if the SNAP-25 promoter is truncated by removal of sequences from -2200 to -288 relative to the transcriptional start site. Moreover, a region of the SNAP-25 promoter from -288 to -126 can render a heterologous promoter responsive to activation by all three Brn-3 factors. Differences in promoter structure may thus result in differences in the response to different Brn-3 factors, thus allowing these factors to produce diverse activation patterns of neuronally expressed genes, such as those encoding different synaptic proteins.
AB - The three members of the Brn-3 subfamily of POU transcription factors have distinct effects on target gene expression. We show that the promoter of the gene encoding the presynaptic nerve terminal protein SNAP-25 resembles previously characterised target genes in being activated by Brn-3a and Brn-3c, but being repressed by Brn-3b. Unlike other target genes, however, the SNAP-25 promoter can be activated by either the N- or C-terminal activation domains of Brn-3a. In contrast to the SNAP-25 gene, the gene encoding the synaptic vesicle protein synapsin I is activated by all the Bm-3 factors, the first gene for which this activation pattern has been reported. Interestingly, however, similar activation by all three Brn-3 factors can be observed if the SNAP-25 promoter is truncated by removal of sequences from -2200 to -288 relative to the transcriptional start site. Moreover, a region of the SNAP-25 promoter from -288 to -126 can render a heterologous promoter responsive to activation by all three Brn-3 factors. Differences in promoter structure may thus result in differences in the response to different Brn-3 factors, thus allowing these factors to produce diverse activation patterns of neuronally expressed genes, such as those encoding different synaptic proteins.
KW - Bm-3
KW - POU family transcription factor
KW - SNAP-25
KW - Synapsin
KW - Transcriptional control
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U2 - 10.1016/S0169-328X(96)00207-0
DO - 10.1016/S0169-328X(96)00207-0
M3 - Article
C2 - 9037543
AN - SCOPUS:0030608508
SN - 0169-328X
VL - 43
SP - 279
EP - 285
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1-2
ER -