Differential regulation of gastrin and somatostatin secretion from isolated perfused rat stomachs

Robert Martindale, Gordon L. Kauffman, Seymour Levin, John H. Walsh, Tadataka Yamada

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

In order to examine the relationships between gut somatostatinlike immunoreactivity and gastrin, we studied the regulation of their secretion from isolated perfused extrinsically denervated rat gut preparations by bombesin nonapeptide and carbamyl choline. Carbamyl choline inhibited somatostatinlike immunoreactivity and stimulated gastrin secretion in a dose-dependent fashion with maximum responses at 10-6 M. These effects of carbamyl choline were abolished by atropine but not by hexamethonium. Conversely, bombesin nonapeptide stimulated both somatostatinlike immunoreactivity and gastrin secretion in doses as low as 10-10 M for somatostatinlike immunoreactivity and 10-9 M for gastrin. Neither atropine nor hexamethonium influenced bombesin-stimulated gastrin release but both agents abolished bombesin-stimulated somatostatinlike immunoreactivity secretion. The combination of carbamyl choline and bombesin affected somatostatinlike immunoreactivity and gastrin secretion in an identical fashion as carbamyl choline alone. These results suggest that somatostatinlike immunoreactivity and gastrin secretion from the gut are differentially regulated. Carbamyl choline stimulates gastrin release and inhibits somatostatin release by activation of muscarinic cholinergic receptors. Bombesin stimulates somatostatin release by activation of neural pathways involving both nicotinic and muscarinic cholinergic receptors. However, stimulation of gastrin release by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells.

Original languageEnglish (US)
Pages (from-to)240-244
Number of pages5
JournalGastroenterology
Volume83
Issue number1 PART 2
DOIs
StatePublished - 1982
Externally publishedYes

Fingerprint

Gastrins
Somatostatin
Stomach
Bombesin
Choline
Neural Pathways
Hexamethonium
Cholinergic Receptors
Muscarinic Receptors
Atropine
Gastrin-Secreting Cells
somatostatin-like peptides
Cholinergic Agents

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Differential regulation of gastrin and somatostatin secretion from isolated perfused rat stomachs. / Martindale, Robert; Kauffman, Gordon L.; Levin, Seymour; Walsh, John H.; Yamada, Tadataka.

In: Gastroenterology, Vol. 83, No. 1 PART 2, 1982, p. 240-244.

Research output: Contribution to journalArticle

Martindale, Robert ; Kauffman, Gordon L. ; Levin, Seymour ; Walsh, John H. ; Yamada, Tadataka. / Differential regulation of gastrin and somatostatin secretion from isolated perfused rat stomachs. In: Gastroenterology. 1982 ; Vol. 83, No. 1 PART 2. pp. 240-244.
@article{fb4de008c39840c8ae31675348f87d05,
title = "Differential regulation of gastrin and somatostatin secretion from isolated perfused rat stomachs",
abstract = "In order to examine the relationships between gut somatostatinlike immunoreactivity and gastrin, we studied the regulation of their secretion from isolated perfused extrinsically denervated rat gut preparations by bombesin nonapeptide and carbamyl choline. Carbamyl choline inhibited somatostatinlike immunoreactivity and stimulated gastrin secretion in a dose-dependent fashion with maximum responses at 10-6 M. These effects of carbamyl choline were abolished by atropine but not by hexamethonium. Conversely, bombesin nonapeptide stimulated both somatostatinlike immunoreactivity and gastrin secretion in doses as low as 10-10 M for somatostatinlike immunoreactivity and 10-9 M for gastrin. Neither atropine nor hexamethonium influenced bombesin-stimulated gastrin release but both agents abolished bombesin-stimulated somatostatinlike immunoreactivity secretion. The combination of carbamyl choline and bombesin affected somatostatinlike immunoreactivity and gastrin secretion in an identical fashion as carbamyl choline alone. These results suggest that somatostatinlike immunoreactivity and gastrin secretion from the gut are differentially regulated. Carbamyl choline stimulates gastrin release and inhibits somatostatin release by activation of muscarinic cholinergic receptors. Bombesin stimulates somatostatin release by activation of neural pathways involving both nicotinic and muscarinic cholinergic receptors. However, stimulation of gastrin release by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells.",
author = "Robert Martindale and Kauffman, {Gordon L.} and Seymour Levin and Walsh, {John H.} and Tadataka Yamada",
year = "1982",
doi = "10.1016/0016-5085(82)90181-0",
language = "English (US)",
volume = "83",
pages = "240--244",
journal = "Gastroenterology",
issn = "0016-5085",
publisher = "W.B. Saunders Ltd",
number = "1 PART 2",

}

TY - JOUR

T1 - Differential regulation of gastrin and somatostatin secretion from isolated perfused rat stomachs

AU - Martindale, Robert

AU - Kauffman, Gordon L.

AU - Levin, Seymour

AU - Walsh, John H.

AU - Yamada, Tadataka

PY - 1982

Y1 - 1982

N2 - In order to examine the relationships between gut somatostatinlike immunoreactivity and gastrin, we studied the regulation of their secretion from isolated perfused extrinsically denervated rat gut preparations by bombesin nonapeptide and carbamyl choline. Carbamyl choline inhibited somatostatinlike immunoreactivity and stimulated gastrin secretion in a dose-dependent fashion with maximum responses at 10-6 M. These effects of carbamyl choline were abolished by atropine but not by hexamethonium. Conversely, bombesin nonapeptide stimulated both somatostatinlike immunoreactivity and gastrin secretion in doses as low as 10-10 M for somatostatinlike immunoreactivity and 10-9 M for gastrin. Neither atropine nor hexamethonium influenced bombesin-stimulated gastrin release but both agents abolished bombesin-stimulated somatostatinlike immunoreactivity secretion. The combination of carbamyl choline and bombesin affected somatostatinlike immunoreactivity and gastrin secretion in an identical fashion as carbamyl choline alone. These results suggest that somatostatinlike immunoreactivity and gastrin secretion from the gut are differentially regulated. Carbamyl choline stimulates gastrin release and inhibits somatostatin release by activation of muscarinic cholinergic receptors. Bombesin stimulates somatostatin release by activation of neural pathways involving both nicotinic and muscarinic cholinergic receptors. However, stimulation of gastrin release by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells.

AB - In order to examine the relationships between gut somatostatinlike immunoreactivity and gastrin, we studied the regulation of their secretion from isolated perfused extrinsically denervated rat gut preparations by bombesin nonapeptide and carbamyl choline. Carbamyl choline inhibited somatostatinlike immunoreactivity and stimulated gastrin secretion in a dose-dependent fashion with maximum responses at 10-6 M. These effects of carbamyl choline were abolished by atropine but not by hexamethonium. Conversely, bombesin nonapeptide stimulated both somatostatinlike immunoreactivity and gastrin secretion in doses as low as 10-10 M for somatostatinlike immunoreactivity and 10-9 M for gastrin. Neither atropine nor hexamethonium influenced bombesin-stimulated gastrin release but both agents abolished bombesin-stimulated somatostatinlike immunoreactivity secretion. The combination of carbamyl choline and bombesin affected somatostatinlike immunoreactivity and gastrin secretion in an identical fashion as carbamyl choline alone. These results suggest that somatostatinlike immunoreactivity and gastrin secretion from the gut are differentially regulated. Carbamyl choline stimulates gastrin release and inhibits somatostatin release by activation of muscarinic cholinergic receptors. Bombesin stimulates somatostatin release by activation of neural pathways involving both nicotinic and muscarinic cholinergic receptors. However, stimulation of gastrin release by bombesin does not involve cholinergic neural pathways and may reflect a direct action on gastrin cells.

UR - http://www.scopus.com/inward/record.url?scp=0020053510&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020053510&partnerID=8YFLogxK

U2 - 10.1016/0016-5085(82)90181-0

DO - 10.1016/0016-5085(82)90181-0

M3 - Article

C2 - 6123464

AN - SCOPUS:0020053510

VL - 83

SP - 240

EP - 244

JO - Gastroenterology

JF - Gastroenterology

SN - 0016-5085

IS - 1 PART 2

ER -