Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat

P. A. Barnett, S. Roman-Goldstein, F. Ramsey, C. I. McCormick, G. Sexton, J. Szumowski, Edward Neuwelt

Research output: Contribution to journalArticle

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Abstract

This study characterized agent differential permeability, three- dimensional tumor volume, and survival in an LX-1 human small celt lung carcinoma intracerebral xenograft model in the nude rat. The percent accessible tissue space (distribution volume) and the permeability x capillary surface product for aminoisobutyric acid (M(r) 103), methotrexate (M(r) 454), dextran 10 (M(r) 10,000), and dextran 70 (M(r) 70,000) were measured between 8 and 16 days after inoculation of tumor. Magnetic resonance imaging and histology were used to quantitate intracerebral tumor volume (mm3). Accessible tissue space (ml/g) and permeability x capillary surface product in intracranial tumor, surrounding brain, and subcutaneous tumor decreased with increasing molecular weight of the agent, regardless of the number of days after inoculation. Accessible tissue space in intracranial tumor increased between 8 and 16 days for all agents except dextran 70. There was little change in the subcutaneous tumor or other tissues with time. Tumor volume calculations from imaging studies correlated with volumetric measurements from histological sections (r2 = 98.5%) and illustrated natural tumor progression (9 to 225 mm3). These results provide a basis for therapeutic design based on differential permeability of specific agents and the ability to quantitatively measure brain tumor volume for accessing tumor response.

Original languageEnglish (US)
Pages (from-to)436-449
Number of pages14
JournalAmerican Journal of Pathology
Volume146
Issue number2
StatePublished - 1995

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Nude Rats
Heterografts
Permeability
Tumor Burden
Carcinoma
Lung
Brain
Dextrans
Neoplasms
Capillary Permeability
Brain Neoplasms
Aminoisobutyric Acids
Tissue Distribution
Methotrexate
Histology
Molecular Weight
Magnetic Resonance Imaging
Survival

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Barnett, P. A., Roman-Goldstein, S., Ramsey, F., McCormick, C. I., Sexton, G., Szumowski, J., & Neuwelt, E. (1995). Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat. American Journal of Pathology, 146(2), 436-449.

Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat. / Barnett, P. A.; Roman-Goldstein, S.; Ramsey, F.; McCormick, C. I.; Sexton, G.; Szumowski, J.; Neuwelt, Edward.

In: American Journal of Pathology, Vol. 146, No. 2, 1995, p. 436-449.

Research output: Contribution to journalArticle

Barnett, PA, Roman-Goldstein, S, Ramsey, F, McCormick, CI, Sexton, G, Szumowski, J & Neuwelt, E 1995, 'Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat', American Journal of Pathology, vol. 146, no. 2, pp. 436-449.
Barnett PA, Roman-Goldstein S, Ramsey F, McCormick CI, Sexton G, Szumowski J et al. Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat. American Journal of Pathology. 1995;146(2):436-449.
Barnett, P. A. ; Roman-Goldstein, S. ; Ramsey, F. ; McCormick, C. I. ; Sexton, G. ; Szumowski, J. ; Neuwelt, Edward. / Differential permeability and quantitative MR imaging of a human lung carcinoma brain xenograft in the nude rat. In: American Journal of Pathology. 1995 ; Vol. 146, No. 2. pp. 436-449.
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