Differential modulation of nucleus accumbens synapses

James M. Brundege, John Williams

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The nucleus accumbens (NAcc) is a brain region involved in functions ranging from motivation and reward to feeding and drug addiction. The NAcc is typically divided into two major subdivisions, the shell and the core. The primary output neurons of both of these areas are medium spiny neurons (MSNs), which are quiescent at rest and depend on the relative input of excitatory and inhibitory synapses to determine when they fire action potentials. These synaptic inputs are, in turn, regulated by a number of neurochemical signaling agents that can ultimately influence information processing in the NAcc. The present study characterized the ability of three major signaling pathways to modulate synaptic transmission in NAcc MSNs and compared this modulation across different synapses within the NAcc. The opioid [Met]5-enkephalin (ME) inhibited excitatory postsynaptic currents (EPSCs) in shell MSNs, an effect mediated primarily by μ-opioid receptors. Forskolin, an activator of adenylyl cyclase, potentiated shell EPSCs. An analysis of miniature EPSCs indicated a primarily presynaptic site of action, although a smaller postsynaptic effect may have also contributed to the potentiation. Adenosine and an adenosine A1-receptor agonist inhibited shell EPSCs, although no significant tonic inhibition by endogenous adenosine was detected. The effects of these signaling agents were then compared across four different synapses in the NAcc: glutamatergic EPSCs and GABAergic inhibitory postsynaptic currents (IPSCs) in both the core and shell subregions. ME inhibited all four of these synapses but produced a significantly greater inhibition of shell IPSCs than the other synapses. Forskolin produced an increase in transmission at each of the synapses tested. However, analysis of miniature IPSCs in the shell showed no sign of a postsynaptic contribution to this potentiation, in contrast to the shell miniature EPSCs. Tonic inhibition of synaptic currents by endogenous adenosine, which was not observed in shell EPSCs, was clearly present at the other three synapses tested. These results indicate that neuromodulation can vary between the different subregions of the NAcc and between the different synapses within each subregion. This may reflect differences in neuronal interconnections and functional roles between subregions and may contribute to the effects of drugs acting on these systems.

Original languageEnglish (US)
Pages (from-to)142-151
Number of pages10
JournalJournal of Neurophysiology
Volume88
Issue number1
StatePublished - 2002

Fingerprint

Nucleus Accumbens
Excitatory Postsynaptic Potentials
Synapses
Inhibitory Postsynaptic Potentials
Adenosine
Neurons
Methionine Enkephalin
Colforsin
Adenosine A1 Receptor Agonists
Aptitude
Opioid Receptors
Reward
Automatic Data Processing
Adenylyl Cyclases
Synaptic Transmission
Opioid Analgesics
Action Potentials
Substance-Related Disorders
Motivation
Brain

ASJC Scopus subject areas

  • Physiology
  • Neuroscience(all)

Cite this

Differential modulation of nucleus accumbens synapses. / Brundege, James M.; Williams, John.

In: Journal of Neurophysiology, Vol. 88, No. 1, 2002, p. 142-151.

Research output: Contribution to journalArticle

Brundege, James M. ; Williams, John. / Differential modulation of nucleus accumbens synapses. In: Journal of Neurophysiology. 2002 ; Vol. 88, No. 1. pp. 142-151.
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