Abstract
Increased prostaglandin E2 (PGE2) synthesis involves multiple enzymes and two isoforms of the terminal enzyme of this biosynthetic pathway, PGE synthase (PGES), were recently identified. Cytosolic PGES (cPGES) is identical to the Hsp90 chaperone, p23, and is reportedly functionally coupled to constitutive PG endoperoxide H synthase-1 (PGHS-1). Microsomal PGES (mPGES), on the other hand, is inducible by proinflammatory cytokines such as IL-1β. We have studied the cellular localization of both enzyme isoforms in human placenta at term and early gestation (10 weeks). Cytosolic PGES was immunolocalized to the fibroblasts and macrophages in villous stroma, whereas mPGES was localized in the extravillous trophoblasts (EVTs) as well as macrophages in both term and early gestation tissues. Microsomal PGES was also observed in cytotrophoblasts (CTs), but not in syncytiotrophoblasts (STs), in early gestation. Apoptotic early gestational STs were heavily stained with cPGES. We also investigated the cellular localization of cPLA2 and PGHS-2 in early gestation and at term. Cytosolic PLA2 was immunolocalized to the stroma and STs at term, but was only observed in CTs in early gestation. PGHS-2, on the other hand, was immunolocalized to both extravillous and STs in early gestation and at term. Our results suggest that mPGES could play a role in trophoblast invasion via its association with EVTs in the basal plate, whereas cPGES could be involved in apoptosis or repair mechanisms.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 259-265 |
| Number of pages | 7 |
| Journal | Placenta |
| Volume | 25 |
| Issue number | 4 |
| DOIs | |
| State | Published - Apr 2004 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Obstetrics and Gynecology
Cite this
Differential localization of prostaglandin E synthase isoforms in human placental cell types. / Meadows, J. W.; Pitzer, B.; Brockman, D. E.; Myatt, Leslie.
In: Placenta, Vol. 25, No. 4, 04.2004, p. 259-265.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Differential localization of prostaglandin E synthase isoforms in human placental cell types
AU - Meadows, J. W.
AU - Pitzer, B.
AU - Brockman, D. E.
AU - Myatt, Leslie
PY - 2004/4
Y1 - 2004/4
N2 - Increased prostaglandin E2 (PGE2) synthesis involves multiple enzymes and two isoforms of the terminal enzyme of this biosynthetic pathway, PGE synthase (PGES), were recently identified. Cytosolic PGES (cPGES) is identical to the Hsp90 chaperone, p23, and is reportedly functionally coupled to constitutive PG endoperoxide H synthase-1 (PGHS-1). Microsomal PGES (mPGES), on the other hand, is inducible by proinflammatory cytokines such as IL-1β. We have studied the cellular localization of both enzyme isoforms in human placenta at term and early gestation (10 weeks). Cytosolic PGES was immunolocalized to the fibroblasts and macrophages in villous stroma, whereas mPGES was localized in the extravillous trophoblasts (EVTs) as well as macrophages in both term and early gestation tissues. Microsomal PGES was also observed in cytotrophoblasts (CTs), but not in syncytiotrophoblasts (STs), in early gestation. Apoptotic early gestational STs were heavily stained with cPGES. We also investigated the cellular localization of cPLA2 and PGHS-2 in early gestation and at term. Cytosolic PLA2 was immunolocalized to the stroma and STs at term, but was only observed in CTs in early gestation. PGHS-2, on the other hand, was immunolocalized to both extravillous and STs in early gestation and at term. Our results suggest that mPGES could play a role in trophoblast invasion via its association with EVTs in the basal plate, whereas cPGES could be involved in apoptosis or repair mechanisms.
AB - Increased prostaglandin E2 (PGE2) synthesis involves multiple enzymes and two isoforms of the terminal enzyme of this biosynthetic pathway, PGE synthase (PGES), were recently identified. Cytosolic PGES (cPGES) is identical to the Hsp90 chaperone, p23, and is reportedly functionally coupled to constitutive PG endoperoxide H synthase-1 (PGHS-1). Microsomal PGES (mPGES), on the other hand, is inducible by proinflammatory cytokines such as IL-1β. We have studied the cellular localization of both enzyme isoforms in human placenta at term and early gestation (10 weeks). Cytosolic PGES was immunolocalized to the fibroblasts and macrophages in villous stroma, whereas mPGES was localized in the extravillous trophoblasts (EVTs) as well as macrophages in both term and early gestation tissues. Microsomal PGES was also observed in cytotrophoblasts (CTs), but not in syncytiotrophoblasts (STs), in early gestation. Apoptotic early gestational STs were heavily stained with cPGES. We also investigated the cellular localization of cPLA2 and PGHS-2 in early gestation and at term. Cytosolic PLA2 was immunolocalized to the stroma and STs at term, but was only observed in CTs in early gestation. PGHS-2, on the other hand, was immunolocalized to both extravillous and STs in early gestation and at term. Our results suggest that mPGES could play a role in trophoblast invasion via its association with EVTs in the basal plate, whereas cPGES could be involved in apoptosis or repair mechanisms.
UR - http://www.scopus.com/inward/record.url?scp=1942455297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=1942455297&partnerID=8YFLogxK
U2 - 10.1016/j.placenta.2003.09.004
DO - 10.1016/j.placenta.2003.09.004
M3 - Article
C2 - 15028417
AN - SCOPUS:1942455297
VL - 25
SP - 259
EP - 265
JO - Placenta
JF - Placenta
SN - 0143-4004
IS - 4
ER -