TY - JOUR
T1 - Differential isoform profiles of α2-macroglobulin from plasma of patients with chronic-progressive or relapsing-remitting multiple sclerosis
AU - Back, Stephen A.
AU - Alhadeff, Jack A.
N1 - Funding Information:
This researchw as supportedi n part by USPHS grant (AM 33532)S. .A. Back is a recipiento f a Bank of America-GianniniF oundationP ostdoctoralF ellowship.W e thank Dr. Stanley van den Noort for support, Maice Apodaca for technical assistanceJo, hn Romine, M.D. and Cathy Romine, R.N. for blood samplesfr om patientsa nd the San Diego chaptero f the National Multiple SclerosisS ocietya nd Jo Ann Leone for locatingp atients.
PY - 1992/10/15
Y1 - 1992/10/15
N2 - Multiple sclerosis (MS) is a human neurological disease for which no clinically useful marker has been identified in blood. This study examined α2-macroglobulin (α2M) from the plasma of six patients with chronic-progressive MS and six with relapsing-remitting disease. The α2M trypsin-binding activity in the plasma from both groups of patients did not differ from normal controls. However, after column isoelectric focusing, consistently less α2M activity was recovered from the MS samples: those from the chronic-progressive and relapsing-remitting disease groups were an average of 43% and 68%, respectively, of controls. The number and isoelectric point (pI) values of the isoforms of the α2M from patients with chronicprogressive disease were similar to controls. The average pI of the major form for both groups was 6.6. By contrast, the average pI of the major form from the patients with relapsing-remitting MS was significantly elevated to 7.1, and this group displayed a significantly higher percentage of total recovered activity above pH 7.0. In eleven of the twelve cases examined, the pI of the major form of α2M correctly correlated with the clinical status of the patient. The original clinical diagnosis of the patients was reassessed by a 9-year retrospective interview which verified that 9 of the 10 patients in the follow-up group retained their original clinical diagnosis. These studies demonstrate differential isoform profiles of native α2M from MS patients with progressive versus remitting disease which may be useful in subclassifying MS patients.
AB - Multiple sclerosis (MS) is a human neurological disease for which no clinically useful marker has been identified in blood. This study examined α2-macroglobulin (α2M) from the plasma of six patients with chronic-progressive MS and six with relapsing-remitting disease. The α2M trypsin-binding activity in the plasma from both groups of patients did not differ from normal controls. However, after column isoelectric focusing, consistently less α2M activity was recovered from the MS samples: those from the chronic-progressive and relapsing-remitting disease groups were an average of 43% and 68%, respectively, of controls. The number and isoelectric point (pI) values of the isoforms of the α2M from patients with chronicprogressive disease were similar to controls. The average pI of the major form for both groups was 6.6. By contrast, the average pI of the major form from the patients with relapsing-remitting MS was significantly elevated to 7.1, and this group displayed a significantly higher percentage of total recovered activity above pH 7.0. In eleven of the twelve cases examined, the pI of the major form of α2M correctly correlated with the clinical status of the patient. The original clinical diagnosis of the patients was reassessed by a 9-year retrospective interview which verified that 9 of the 10 patients in the follow-up group retained their original clinical diagnosis. These studies demonstrate differential isoform profiles of native α2M from MS patients with progressive versus remitting disease which may be useful in subclassifying MS patients.
KW - Isoelectric focusing
KW - Multiple sclerosis
KW - α-Macroglobulin
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U2 - 10.1016/0009-8981(92)90102-V
DO - 10.1016/0009-8981(92)90102-V
M3 - Article
C2 - 1281762
AN - SCOPUS:0026463023
SN - 0009-8981
VL - 211
SP - 27
EP - 36
JO - Clinica Chimica Acta
JF - Clinica Chimica Acta
IS - 1-2
ER -