Differential gene expression in the lens epithelial cells from selenite injected rats

Takeshi Nakajima, Emi Nakajima, Chiho Fukiage, Mitsuyoshi Azuma, Thomas R. Shearer

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

The mechanism causing loss of integrity of lens epithelial cells induced by an overdose of sodium selenite remains to be elucidated. The aim of the present experiment was to search for changes in gene expression in epithelial cells of lenses from rats developing selenite cataract. One day after injection of selenite into 12 day old rats, gene expression in lens epithelial cells was analysed using a commercial DNA array (Atlas Rat 1.2 Array). Changes were confirmed by RT-PCR. Of 1176 genes assayed by hybridization, 91 genes showed differences in expression between normal and selenite lenses. The three genes showing the greatest changes were: cytochrome c oxidase subunit I (COX-I, decrease), gastric inhibitory polypeptide (GIP, decrease), and early growth response protein-1 (EGR-1, increase). Both COX-I and EGR-1 have been reported to be involved with apoptosis. These results suggest that changes in COX-I and EGR-1 expression in lens epithelial cells might play important roles in apoptosis and altered metabolism leading to selenite cataract.

Original languageEnglish (US)
Pages (from-to)231-236
Number of pages6
JournalExperimental Eye Research
Volume74
Issue number2
DOIs
StatePublished - Jan 1 2002

Keywords

  • COX-I
  • DNA array
  • EGR-1
  • GIP
  • Lens epithelium
  • Selenite

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

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