Differential gene expression between neuropeptide y expressing neurons of the dorsomedial nucleus of the Hypothalamus and the Arcuate Nucleus: Microarray Analysis Study

Shin Draper, Melissa Kirigiti, Maria Glavas, Bernadette Grayson, C. N.Angie Chong, Betty Jiang, M. Susan Smith, Lori M. Zeltser, Kevin L. Grove

    Research output: Contribution to journalArticle

    24 Scopus citations

    Abstract

    The Dorsomedial Nucleus of the Hypothalamus (DMH) is known to play important roles in ingestive behavior and body weight homeostasis. The DMH contains neurons expressing Neuropeptide Y (NPY) during specific physiological conditions of hyperphagia and obesity, however, the role of DMH-NPY neurons has yet to be characterized. In contrast to the DMH-NPY neurons, NPY expressing neurons have been best characterized in the Arcuate Nucleus of the Hypothalamus (ARH). The purpose of this study is to characterize the chemical phenotype of DMH-NPY neurons by comparing the gene expression profiles of NPY neurons in the DMH and ARH isolated from postnatal NPY-hrGFP mice by microarray analysis. Twenty genes were differentially expressed in the DMH-NPY neurons compared to the ARH. Among them, there were several transcriptional factors that play important roles in the regulation of energy balance. DMH-NPY neurons expressed Glutamic Acid Decarboxylase (GAD) 65 and 67, suggesting that they may be GABAergic, similar to ARH-NPY neurons. While ARH-NPY neurons expressed leptin receptor (ObRb) and displayed the activation of STAT3 in response to leptin administration, DMH-NPY neurons showed neither. These findings strongly suggest that DMH-NPY neurons could play a distinct role in the control of energy homeostasis and are differentially regulated from ARH-NPY neurons through afferent inputs and transcriptional regulators.

    Original languageEnglish (US)
    Pages (from-to)139-150
    Number of pages12
    JournalBrain research
    Volume1350
    DOIs
    StatePublished - Sep 2 2010

    Keywords

    • Development
    • Hypothalamus
    • Leptin
    • Neuropeptide Y
    • Transcription factors

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Molecular Biology
    • Clinical Neurology
    • Developmental Biology

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