Differential expression of voltage-gated calcium channels in identified visual cortical neurons

Kelleen Giffin, Joel Solomon, Andreas Burkhalter, Jeanne M. Nerbonne

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Using the whole-cell patch-clamp technique, Ca2+ channel currents were examined in three distinct types of neurons derived from rat primary visual cortex. Callosal-projecting and superior coil iculus-projecting neurons were identified following in vivo retrograde labeling with fluorescent "beads." A subset of intrinsic GABAergic visual cortical neurons was identified with the monoclonal antibody VC1.1. Although high voltage activated Ca2+ channel currents were measured in all three cell types, clear differences in the densities of these channels were observed. There were also marked variations in the relative amplitudes of the inactivating and noninactivating components of the high voltage-activated currents, suggesting that N- and L-type Ca2+ channels are differentially distributed. Although low voltage activated or T-type currents were measured in subsets of both types of projection neurons, they were not observed in VC1.1-positive cells. These results provide a direct demonstration that voltage-gated Ca2+ channels are expressed in neurons of the mammalian visual cortex and reveal that the distributions and densities of different Ca2+ channel types in diverse classes of visual cortical neurons are distinct.

Original languageEnglish (US)
Pages (from-to)321-332
Number of pages12
JournalNeuron
Volume6
Issue number3
DOIs
StatePublished - 1991
Externally publishedYes

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Calcium Channels
Neurons
Visual Cortex
Corpus Callosum
Patch-Clamp Techniques
Monoclonal Antibodies

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Differential expression of voltage-gated calcium channels in identified visual cortical neurons. / Giffin, Kelleen; Solomon, Joel; Burkhalter, Andreas; Nerbonne, Jeanne M.

In: Neuron, Vol. 6, No. 3, 1991, p. 321-332.

Research output: Contribution to journalArticle

Giffin, Kelleen ; Solomon, Joel ; Burkhalter, Andreas ; Nerbonne, Jeanne M. / Differential expression of voltage-gated calcium channels in identified visual cortical neurons. In: Neuron. 1991 ; Vol. 6, No. 3. pp. 321-332.
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