Differential expression of RANTES chemokine, TGF-β, and leukocyte phenotype in acute cellular rejection and quilty B lesions

Paul J. Michaels, Jon Kobashigawa, Hillel Laks, Amir Azarbal, Maria L. Espejo, Leslie Chen, Michael C. Fishbein

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Because of the complexity of the trabeculated endocardial surface and tangential histologic sectioning, the differentiation of acute cellular rejection (ACR) from Quilty B lesions (QB) in endomyocardial biopsies (EMBs) is problematic. We hypothesized that the phenotype chemokine RANTES (regulated upon activation, normal T cell expressed and secreted) expression of infiltrating cells and the pattern of expression of transforming growth factor-β (TGF-β) may distinguish ACR from QB. In previous studies, the number of RANTES-positive cells and the expression of TGF-β correlated with the severity of rejection. Methods: We used immunohistochemical techniques to stain sections of human EMBs with only QB (n = 14) or with only ACR (International Society for Heart and Lung Transplantation Grades 1A and 1B, n = 7; Grades 3A and 3B, n = 7) for B (CD20) and T-lymphocytes (CD3), macrophages (CD68), RANTES, and TGF-β expression. We graded the percentage of positive cells from 0 to 4 (1 = 1% to 25%; 2 = 26% to 50%; 3 = 51% to 75%, and 4 = 76% to 100%). Results: When ACR was compared with QB, we found no difference in the proportion of myocardial B cells (0.9 ± 0.3 vs 1.1 ± 0.3, p = 0.17); however, we found a lesser proportion of T cells (1.8 ± 0.5 vs 2.8 ± 0.9, p

Original languageEnglish (US)
Pages (from-to)407-416
Number of pages10
JournalJournal of Heart and Lung Transplantation
Volume20
Issue number4
DOIs
StatePublished - 2001
Externally publishedYes

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Transforming Growth Factors
Chemokines
Leukocytes
T-Lymphocytes
Phenotype
Biopsy
B-Lymphocytes
Coloring Agents
Macrophages

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery
  • Transplantation

Cite this

Differential expression of RANTES chemokine, TGF-β, and leukocyte phenotype in acute cellular rejection and quilty B lesions. / Michaels, Paul J.; Kobashigawa, Jon; Laks, Hillel; Azarbal, Amir; Espejo, Maria L.; Chen, Leslie; Fishbein, Michael C.

In: Journal of Heart and Lung Transplantation, Vol. 20, No. 4, 2001, p. 407-416.

Research output: Contribution to journalArticle

Michaels, Paul J. ; Kobashigawa, Jon ; Laks, Hillel ; Azarbal, Amir ; Espejo, Maria L. ; Chen, Leslie ; Fishbein, Michael C. / Differential expression of RANTES chemokine, TGF-β, and leukocyte phenotype in acute cellular rejection and quilty B lesions. In: Journal of Heart and Lung Transplantation. 2001 ; Vol. 20, No. 4. pp. 407-416.
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