Differential expression of progestin receptor isoforms in the hypothalamus, pituitary, and endometrium of rhesus macaques

Cynthia Bethea, Andrea A. Widmann

    Research output: Contribution to journalArticle

    52 Citations (Scopus)

    Abstract

    The progestin receptor exists in at least two isoforms: a long form (PR- B) and a short form (PR-A), which can be separated and detected with Western blot analysis. It has been suggested from in vitro transfection experiments that differential expression of the two isoforms may provide one mechanism for tissue specific actions of progesterone (P). However, more information from in vivo experimentation is needed. It has been reported that P down- regulates the expression of PR in the endometrium and pituitary of E primed macaques. However, PR protein and PR messenger RNA expression in the hypothalamus is maintained with P treatment of E-primed macaques. Thus, there is tissue-specific regulation of PR by its cognate ligand in the nonhuman primate. To gain insight into the tissue-specific regulation of PR by P, we questioned whether differential expression of the isoforms of PR exists in the endometrium, pituitary, and hypothalamus of rhesus monkeys. The expression of PR-A and PR-B was examined after E (28-30 days) and E + P (14 days E + 14 days E + P) treatment in the primate endometrium, pituitary, and hypothalamus. After E or E + P treatment, the levels of PR-A were 5 times higher than PR-B in the endometrium. PR-A was 1.6-fold higher than PR-B in the pituitary. In the hypothalamus, the ratio of A to B ranged from less than 1 (B exceeds A) to unity (A and B equimolar). There was no difference in the ratio of A to B between E-treated and E + P-treated groups in any tissue examined. These observations (a) provide further support of the hypothesis that differential expression of the isoforms of PR may subserve the tissue specific actions of P and (b) also suggest that P does not differentially affect the expression of the isoforms of its cognate receptor in the endometrium, pituitary, or hypothalamus.

    Original languageEnglish (US)
    Pages (from-to)677-687
    Number of pages11
    JournalEndocrinology
    Volume139
    Issue number2
    DOIs
    StatePublished - 1998

    Fingerprint

    Progesterone Receptors
    Endometrium
    Macaca mulatta
    Hypothalamus
    Protein Isoforms
    Macaca
    Primates
    Transfection
    Progesterone
    Down-Regulation
    Western Blotting
    Ligands
    Messenger RNA
    Proteins

    ASJC Scopus subject areas

    • Endocrinology
    • Endocrinology, Diabetes and Metabolism

    Cite this

    Differential expression of progestin receptor isoforms in the hypothalamus, pituitary, and endometrium of rhesus macaques. / Bethea, Cynthia; Widmann, Andrea A.

    In: Endocrinology, Vol. 139, No. 2, 1998, p. 677-687.

    Research output: Contribution to journalArticle

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