Differential expression of insulin-like growth factor genes in rat central nervous system

P. Rotwein, S. K. Burgess, J. D. Milbrandt, J. E. Krause

Research output: Contribution to journalArticle

379 Citations (Scopus)

Abstract

A sensitive solution-hybridization assay was used to investigate the expression of genes encoding insulin-like growth factors I and II (IGF-I and -II) in the rat central nervous system (CNS). mRNAs for both IGFs are synthesized throughout the CNS of adult rats but exhibit distinct regional differences for each growth factor. IGF-I mRNA is 8-10 times more abundant in the cervical-thoracic spinal cord and in the olfactory bulb than in whole brain and is enriched 3-fold in the midbrain and cerebellum. IGF-II mRNA is minimally enriched in the medulla-pons and cerebellum but is 3-5 times less abundant in the midbrain and corpus striatum than in total brain. During CNS development the content of IGF-I and IGF-II mRNAs is highest at embryonic day 14 and declines by a factor of 3-4 at birth, to values found in adult brain. Embryonic neurons and glia synthesize IGF-I mRNA during short-term cell culture; only glia produce IGF-II mRNA. These observations show that IGF-I and IGF-II are differentially expressed in the developing and adult CNS and suggest that each growth factor may play a unique role in the mammalian nervous system.

Original languageEnglish (US)
Pages (from-to)265-269
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume85
Issue number1
StatePublished - 1988
Externally publishedYes

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Insulin-Like Growth Factor II
Somatomedins
Insulin-Like Growth Factor I
Central Nervous System
Messenger RNA
Genes
Mesencephalon
Neuroglia
Cerebellum
Intercellular Signaling Peptides and Proteins
Brain
Corpus Striatum
Pons
Olfactory Bulb
Nervous System
Thorax
Cell Culture Techniques
Parturition
Gene Expression
Neurons

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

Differential expression of insulin-like growth factor genes in rat central nervous system. / Rotwein, P.; Burgess, S. K.; Milbrandt, J. D.; Krause, J. E.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 85, No. 1, 1988, p. 265-269.

Research output: Contribution to journalArticle

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