Differential expression of cytokine genes in monocytes, peritoneal macrophages and liver following endotoxin- or turpentine-induced inflammation in rat

M. Scotté, M. Hiron, S. Masson, S. Lyoumi, Fatima Banine, P. Ténière, J. P. Lebreton, M. Daveau

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

Pro-inflammatory cytokines are produced after systemic or local inflammation by a wide variety of cell types including monocytes, macrophages, Kupffer and endothelial cells. Previous studies have shown that IL-6 gene expression does not occur in liver from rats undergoing an acute phase response after turpentine injection or controls. These data do not rule out the possibility that delivery of a pathogen to the liver via the portal circulation could directly activate the Kupffer cells. Rats were injected either intravenously or intraperitoneally with LPS, or subcutaneously with turpentine oil. The changes in IL-1β, IL-6, and TNF mRNA levels in monocytes (collected from portal vein or caval cein), peritoneal macrophages and liver over a 3-hour period post-treatment were examined. The kinetics of LPS-vs turpentine-induced cytokine mRNAs in these various cell types were compared by quantitative reverse transcription and polymerase chain reaction (RT-PCR). Our data demonstrate that an intrahepatic expression of cytokines in the non parenchymal cells was induced by an LPS challenge but not by a turpentine-induced inflammation. This process could act as a paracrine mechanism in the acute-phase response and play a role in the modulation of hepatic regeneration.

Original languageEnglish (US)
Pages (from-to)115-120
Number of pages6
JournalCytokine
Volume8
Issue number2
DOIs
StatePublished - Feb 1996
Externally publishedYes

Fingerprint

Turpentine
Macrophages
Peritoneal Macrophages
Endotoxins
Liver
Rats
Monocytes
Genes
Cytokines
Inflammation
Gene Expression
Acute-Phase Reaction
Kupffer Cells
Interleukin-6
Messenger RNA
Venae Cavae
Polymerase chain reaction
Endothelial cells
Pathogens
Transcription

Keywords

  • Cytokine
  • Endotoxaemia
  • Inflammation
  • RT-PCR

ASJC Scopus subject areas

  • Endocrinology
  • Molecular Biology
  • Immunology
  • Immunology and Allergy

Cite this

Differential expression of cytokine genes in monocytes, peritoneal macrophages and liver following endotoxin- or turpentine-induced inflammation in rat. / Scotté, M.; Hiron, M.; Masson, S.; Lyoumi, S.; Banine, Fatima; Ténière, P.; Lebreton, J. P.; Daveau, M.

In: Cytokine, Vol. 8, No. 2, 02.1996, p. 115-120.

Research output: Contribution to journalArticle

Scotté, M. ; Hiron, M. ; Masson, S. ; Lyoumi, S. ; Banine, Fatima ; Ténière, P. ; Lebreton, J. P. ; Daveau, M. / Differential expression of cytokine genes in monocytes, peritoneal macrophages and liver following endotoxin- or turpentine-induced inflammation in rat. In: Cytokine. 1996 ; Vol. 8, No. 2. pp. 115-120.
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