Differential expression of collagenase by human fibroblasts and bone marrow stromal cells

Gary Takahashi, Dawn Moran, D. Frank Andrews, Jack W. Singer

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The bone marrow stroma, represented in long-term marrow culture by cells of the adherent layer, is composed of a heterogenous mixture of macrophages and mesenchymal cells, including fibroblasts, endothelial cells and adipocytes, in association with a proteoglycan matrix. This matrix, which is synthesized by the stroma, is capable of binding hematopoietic growth factors, and likely plays a major role in hematopoietic regulation. Clonally-derived non-transformed bone marrow stromal cells, propagated in the presence of basic fibroblast growth factor, were studied for expression of collagenase, an enzyme whose substrate, collagen, is a major component of the extracellular matrix. Expression of steady-state collagenase mRNA was undetectable in both unstimulated dermal fibroblasts and non-transformed marrow stromal cells. However, stimulation with interleukin 1α (10 U/ml) for 24h resulted in marked accumulation of collagenase mRNA in dermal fibroblast cells, yet failed to elicit a similar response in bone marrow stromal cells. Both marrow stromal cells and dermal fibroblasts constitutively expressed transcripts of collagen I, and rhIL-1α upregulated transcripts of interleukin 6 in both these cells as well. Although similar in morphology, these data indicate that bone marrow stromal cells differ from fibroblasts in their response to IL-1. In the marrow microenvironment, where IL-1 may be secreted by a variety of cell types, such suppression of collagenase expression may serve to prevent unwanted mobilization of collagen from the glycoprotein matrix by marrow stromal cells.

Original languageEnglish (US)
Pages (from-to)305-308
Number of pages4
JournalLeukemia
Volume8
Issue number2
StatePublished - Feb 1994
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 8
Mesenchymal Stromal Cells
Bone Marrow
Collagenases
Fibroblasts
Stromal Cells
Interleukin-1
Collagen
Skin
Messenger RNA
Fibroblast Growth Factor 2
Proteoglycans
Adipocytes
Interleukin-10
Extracellular Matrix
Interleukin-6
Intercellular Signaling Peptides and Proteins
Glycoproteins
Endothelial Cells
Cell Culture Techniques

ASJC Scopus subject areas

  • Hematology
  • Cancer Research

Cite this

Differential expression of collagenase by human fibroblasts and bone marrow stromal cells. / Takahashi, Gary; Moran, Dawn; Andrews, D. Frank; Singer, Jack W.

In: Leukemia, Vol. 8, No. 2, 02.1994, p. 305-308.

Research output: Contribution to journalArticle

Takahashi, G, Moran, D, Andrews, DF & Singer, JW 1994, 'Differential expression of collagenase by human fibroblasts and bone marrow stromal cells', Leukemia, vol. 8, no. 2, pp. 305-308.
Takahashi, Gary ; Moran, Dawn ; Andrews, D. Frank ; Singer, Jack W. / Differential expression of collagenase by human fibroblasts and bone marrow stromal cells. In: Leukemia. 1994 ; Vol. 8, No. 2. pp. 305-308.
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