Differential effects of the hydrophobic surfactant proteins on the formation of inverse bicontinuous cubic phases

Mariya Chavarha, Ryan W. Loney, Kamlesh Kumar, Shankar B. Rananavare, Stephen (Steve) Hall

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Prior studies have shown that the biological mixture of the two hydrophobic surfactant proteins, SP-B and SP-C, produces faster adsorption of the surfactant lipids to an air/water interface, and that they induce 1-palmitoyl-2-oleoyl phosphatidylethanolamine (POPE) to form inverse bicontinuous cubic phases. Previous studies have shown that SP-B has a much greater effect than SP-C on adsorption. If the two proteins induce faster adsorption and formation of the bicontinuous structures by similar mechanisms, then they should also have different abilities to form the cubic phases. To test this hypothesis, we measured small-angle X-ray scattering on the individual proteins combined with POPE. SP-B replicated the dose-related ability of the combined proteins to induce the cubic phases at temperatures more than 25 °C below the point at which POPE alone forms the curved inverse-hexagonal phase. With SP-C, diffraction from cubic structures was either absent or present at very low intensities only with larger amounts of protein. The correlation between the structural effects of inducing curved structures and the functional effects on the rate of adsorption fits with the model in which SP-B promotes adsorption by facilitating formation of an inversely curved, rate-limiting structure.

Original languageEnglish (US)
Pages (from-to)16596-16604
Number of pages9
JournalLangmuir
Volume28
Issue number48
DOIs
StatePublished - Dec 4 2012

Fingerprint

Surface-Active Agents
Surface active agents
surfactants
proteins
Proteins
Adsorption
adsorption
Pulmonary Surfactant-Associated Protein B
X ray scattering
Lipids
lipids
Diffraction
dosage
Water
air
Air
scattering
diffraction
water
phosphatidylethanolamine

ASJC Scopus subject areas

  • Electrochemistry
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Materials Science(all)
  • Spectroscopy

Cite this

Differential effects of the hydrophobic surfactant proteins on the formation of inverse bicontinuous cubic phases. / Chavarha, Mariya; Loney, Ryan W.; Kumar, Kamlesh; Rananavare, Shankar B.; Hall, Stephen (Steve).

In: Langmuir, Vol. 28, No. 48, 04.12.2012, p. 16596-16604.

Research output: Contribution to journalArticle

Chavarha, Mariya ; Loney, Ryan W. ; Kumar, Kamlesh ; Rananavare, Shankar B. ; Hall, Stephen (Steve). / Differential effects of the hydrophobic surfactant proteins on the formation of inverse bicontinuous cubic phases. In: Langmuir. 2012 ; Vol. 28, No. 48. pp. 16596-16604.
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