Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons

T. Esch, V. Lemmon, Gary Banker

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

A fundamental step in neuronal development is the acquisition of a polarized form, with distinct axons and dendrites. Although the ability to develop a polarized form appears to be largely an intrinsic property of neurons, it can be influenced by environmental cues. For example, in cell cultures substrate and diffusible factors can enhance and orient axonal development. In this study we examine the effects of growth on each of two cell adhesion molecules (CAMs), NgCAM and N-cadherin, on the development of polarity by cultured hippocampal neurons. We find that although the same pattern of development occurs on control substrates and the CAMs, the CAMs greatly accelerate the rate and extent of development of axons - axons form sooner and grow longer on the CAMs than on the control substrate. In contrast, the CAMs have opposite effects on dendritic development - N-cadherin enhances, but NgCAM reduces dendritic growth compared to control. These results provide further evidence that the development of polarity is largely determined by a cell-autonomous program, but that environmental cues can independently regulate axonal and dendritic growth.

Original languageEnglish (US)
Pages (from-to)215-223
Number of pages9
JournalJournal of Neurocytology
Volume29
Issue number3
StatePublished - 2000

Fingerprint

Cell Adhesion Molecules
Cadherins
Dendrites
Axons
Neurons
Body Patterning
Cues
Growth
Aptitude
Cell Culture Techniques

ASJC Scopus subject areas

  • Neuroscience(all)
  • Anatomy
  • Cell Biology
  • Histology

Cite this

Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons. / Esch, T.; Lemmon, V.; Banker, Gary.

In: Journal of Neurocytology, Vol. 29, No. 3, 2000, p. 215-223.

Research output: Contribution to journalArticle

@article{9a5d045badfd4383901d133ed885ad89,
title = "Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons",
abstract = "A fundamental step in neuronal development is the acquisition of a polarized form, with distinct axons and dendrites. Although the ability to develop a polarized form appears to be largely an intrinsic property of neurons, it can be influenced by environmental cues. For example, in cell cultures substrate and diffusible factors can enhance and orient axonal development. In this study we examine the effects of growth on each of two cell adhesion molecules (CAMs), NgCAM and N-cadherin, on the development of polarity by cultured hippocampal neurons. We find that although the same pattern of development occurs on control substrates and the CAMs, the CAMs greatly accelerate the rate and extent of development of axons - axons form sooner and grow longer on the CAMs than on the control substrate. In contrast, the CAMs have opposite effects on dendritic development - N-cadherin enhances, but NgCAM reduces dendritic growth compared to control. These results provide further evidence that the development of polarity is largely determined by a cell-autonomous program, but that environmental cues can independently regulate axonal and dendritic growth.",
author = "T. Esch and V. Lemmon and Gary Banker",
year = "2000",
language = "English (US)",
volume = "29",
pages = "215--223",
journal = "Journal of Neurocytology",
issn = "0300-4864",
publisher = "Kluwer Academic Publishers",
number = "3",

}

TY - JOUR

T1 - Differential effects of NgCAM and N-cadherin on the development of axons and dendrites by cultured hippocampal neurons

AU - Esch, T.

AU - Lemmon, V.

AU - Banker, Gary

PY - 2000

Y1 - 2000

N2 - A fundamental step in neuronal development is the acquisition of a polarized form, with distinct axons and dendrites. Although the ability to develop a polarized form appears to be largely an intrinsic property of neurons, it can be influenced by environmental cues. For example, in cell cultures substrate and diffusible factors can enhance and orient axonal development. In this study we examine the effects of growth on each of two cell adhesion molecules (CAMs), NgCAM and N-cadherin, on the development of polarity by cultured hippocampal neurons. We find that although the same pattern of development occurs on control substrates and the CAMs, the CAMs greatly accelerate the rate and extent of development of axons - axons form sooner and grow longer on the CAMs than on the control substrate. In contrast, the CAMs have opposite effects on dendritic development - N-cadherin enhances, but NgCAM reduces dendritic growth compared to control. These results provide further evidence that the development of polarity is largely determined by a cell-autonomous program, but that environmental cues can independently regulate axonal and dendritic growth.

AB - A fundamental step in neuronal development is the acquisition of a polarized form, with distinct axons and dendrites. Although the ability to develop a polarized form appears to be largely an intrinsic property of neurons, it can be influenced by environmental cues. For example, in cell cultures substrate and diffusible factors can enhance and orient axonal development. In this study we examine the effects of growth on each of two cell adhesion molecules (CAMs), NgCAM and N-cadherin, on the development of polarity by cultured hippocampal neurons. We find that although the same pattern of development occurs on control substrates and the CAMs, the CAMs greatly accelerate the rate and extent of development of axons - axons form sooner and grow longer on the CAMs than on the control substrate. In contrast, the CAMs have opposite effects on dendritic development - N-cadherin enhances, but NgCAM reduces dendritic growth compared to control. These results provide further evidence that the development of polarity is largely determined by a cell-autonomous program, but that environmental cues can independently regulate axonal and dendritic growth.

UR - http://www.scopus.com/inward/record.url?scp=0033637615&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033637615&partnerID=8YFLogxK

M3 - Article

C2 - 11428051

AN - SCOPUS:0033637615

VL - 29

SP - 215

EP - 223

JO - Journal of Neurocytology

JF - Journal of Neurocytology

SN - 0300-4864

IS - 3

ER -