Differential effects of insulin-like growth factor (IGF)-binding protein-3 and its proteolytic fragments on ligand binding, cell surface association, and IGF-I receptor signaling

G. R. Devi, D. H. Yang, R. G. Rosenfeld, Y. Oh

Research output: Contribution to journalArticle

33 Scopus citations

Abstract

Insulin-like growth factor (IGF)-binding protein-3 (IGFBP-3), the predominant IGF carrier protein in circulation, is posttranslationally modified in vivo by IGFBP-3 protease(s) into a number of fragments. Based on the ascertained and predicted recognition sites for known IGFBP-3 proteases, FLAG-epitope tagged intact IGFBP-3, NH2-terminal ((1-97)), intermediate fragment ((88-148)), and COOH-terminal fragments ((98-264)) and ((184-264)) were generated in a baculovirus and/or Escherichia coli expression system and examined, by Western ligand blot and affinity cross-linking assays, for their ability to bind IGF and insulin. The NH2- and COOH-terminal fragments bound both IGF and insulin specifically (albeit with significantly reduced affinity) for IGF but higher affinity for insulin, when compared with intact IG-FBP-3. The effect of IGFBP-3 and the fragments on IGF-I receptor (IGFIR) signaling pathways was studied by testing IGF-I-induced receptor autophosphorylation in IGFIR-overexpressing NIH-3T3 cells. IGFBP-3 showed a dose-dependent inhibition of autophosphorylation of the β-subunit of IGFIR. The ((1-97)) NH2-terminal fragment inhibited IGFIR autophosphorylation at high concentrations, and this effect seems largely attributable to sequestration of IGF-I. In contrast, no inhibition of IGF-I-induced IGFIR autophosphorylation was detectable with the ((98-264)) and ((184-264)) COOH-terminal fragments, despite their ability to bind IGF. However, unlike the ((1-97))NH2-terminal fragment, the COOH-terminal fragments of IGFBP-3 retained their ability to associate with the cell surface, and this binding was competed by heparin, similar to intact IGFBP-3.

Original languageEnglish (US)
Pages (from-to)4171-4179
Number of pages9
JournalEndocrinology
Volume141
Issue number11
DOIs
StatePublished - Jan 1 2000

ASJC Scopus subject areas

  • Endocrinology

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