Differential E-selectin expression by iris versus retina microvascular endothelial cells cultured from the same individuals

Matthew D. Silverman, Bobby Babra, Yuzhen Pan, Stephen R. Planck, James T. Rosenbaum

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The microvasculature of the eye plays a critical role in many ophthalmic diseases, including diabetic retinopathy and macular degeneration. Transcriptional profiling by gene array allows characterization of endothelial cells (EC) and can test for inherent EC diversity relative to the tissue source of the EC. Here, we established highly purified microvascular EC cultures from donor-matched human irises and retinae (4 donor pairs). We used nylon-based gene array kits to compare gene expression in paired confluent EC monolayers, under both quiescent and inflammatory agent (bacterial lipopolysaccharide, LPS; or tumor necrosis-factor alpha, TNFα)-activated conditions. In the absence of an inflammatory agent, iris and retinal ECs from the same donor were remarkably similar in overall gene expression profiles, except for possible differences in the expression of platelet-derived growth factor-A and a DNA mismatch repair protein (mutL homologue). Several detectable transcripts had never previously been reported in the eye. After inflammatory stimulation, significantly greater expression of the adhesion molecule E-selectin mRNA was consistently detected in retinal versus iris EC, and this difference was maintained at the protein level both in cell-surface-expressed and secreted soluble E-selectin protein in paired cultures. Thus, cultured EC derived from adjacent microvascular beds are biologically distinct. Such endothelial diversity could play a role in the pathogenesis of tissue-specific inflammation, infection, neovascularization, and malignancy.

Original languageEnglish (US)
Pages (from-to)32-42
Number of pages11
JournalMicrovascular Research
Issue number1-2
StatePublished - Jul 2005


  • Cell adhesion molecule
  • Chemokine
  • E-selectin
  • Endothelium
  • Expression profiling
  • Heterogeneity
  • Microcirculation
  • Ocular inflammation
  • Tumor necrosis factor-α
  • cDNA array

ASJC Scopus subject areas

  • Biochemistry
  • Cardiology and Cardiovascular Medicine
  • Cell Biology


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