Differential Coupling of D1 and D5 Dopamine Receptors to Guanine Nucleotide Binding Proteins in Transfected GH4C1 Rat Somatomammotrophic Cells

Kazuhiro Kimura, Shifra Sela, Claudia Bouvier, David K. Grandy, Anita Sidhu

    Research output: Contribution to journalArticle

    41 Scopus citations

    Abstract

    Abstract: D1 and D5 dopamine receptor genes, stably expressed in GH4C1 rat somatomammotrophic cells, display identical binding values and stimulate adenylate cyclase. Approximately 60% of D1 receptors were in the agonist high‐affinity state and were converted to the low‐affinity state by 100 µM guanyl‐5′‐ylimidodiphosphate [Gpp(NH)p]. Of the 48% of D5 receptors in the high‐affinity state, only half were modulated by 100 µM Gpp(NH)p; in the presence of the G protein activator, AlF4, the high‐affinity sites of D5 receptors were abolished by Gpp(NH)p, suggesting tight coupling between D5 receptors and G proteins. The high‐affinity sites of D1, but not D5, receptors were reduced after pertussis toxin treatment of cells. Thus, whereas D1 receptors in GH4C1 cells couple to both Gs, the G stimulatory protein, and a pertussis toxin‐sensitive G protein, D5 receptors couple to Gs and a pertussis toxin‐insensitive G protein. Neither D1 nor D5 receptors were able to stimulate phosphoinositide metabolism in these cells. The ability of D5, but not D1, receptors to couple to novel G proteins may be significant in assigning a functional role for these receptors.

    Original languageEnglish (US)
    Pages (from-to)2118-2124
    Number of pages7
    JournalJournal of Neurochemistry
    Volume64
    Issue number5
    DOIs
    StatePublished - May 1995

    Keywords

    • Cell transfection
    • Dopamine receptor
    • G protein
    • High‐affinity binding sites
    • Pertussis toxin

    ASJC Scopus subject areas

    • Biochemistry
    • Cellular and Molecular Neuroscience

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