Differential calcium requirements for growth of mouse skin epithelial and fibroblast cells

Molly Kulesz-Martin, D. Fabian, J. S. Bertram

Research output: Contribution to journalArticle

Abstract

Concentrations of extracellular Ca++ optimum for growth of cell types of mesodermal origin have been reported to be up to l00-fold higher than concentrations optimal for epidermal or other epithelial lining cells. In order to examine Ca++ requirements of epithelial v. fibroblastic cells derived from a common tissue source, prior to prolonged culture, freshly isolated mouse epidermal keratinocytes, hair follicle cells and dermal fibroblasts were plated at high density or at clonal density in medium ranging from 0·0l4 to l·4 mM Ca++. Epithelial skin cells grew best at Ca++ levels below 0·l mM while dermal fibroblasts grew best at a Ca++ concentration of l·4 mM. The epithelial cell types exhibited marked morphologic changes in response to Ca++, while the fibroblasts did not. These results suggest that the variations in Ca++ response between lining epithelium and mesenchymal cells resulted from inherent differences in these cell types, but a mechanism for such differential effects has not yet been defined.

Original languageEnglish (US)
Pages (from-to)525-533
Number of pages9
JournalCell and Tissue Kinetics
Volume17
Issue number5
StatePublished - 1984
Externally publishedYes

Fingerprint

Fibroblasts
Epithelial Cells
Calcium
Skin
Growth
Hair Follicle
Keratinocytes
Epithelium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Differential calcium requirements for growth of mouse skin epithelial and fibroblast cells. / Kulesz-Martin, Molly; Fabian, D.; Bertram, J. S.

In: Cell and Tissue Kinetics, Vol. 17, No. 5, 1984, p. 525-533.

Research output: Contribution to journalArticle

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