Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor

P. J. O'hara; T. L. Gilbert; B. A. Haldeman; K. M. Houamed; J. L. Kuijper; F. S. Hagen; W. Almers; E. Mulvihill

doi:10.3109/10425179309015623

Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor

P. J. O'hara, T. L. Gilbert, B. A. Haldeman, K. M. Houamed, J. L. Kuijper, F. S. Hagen, W. Almers, E. Mulvihill

Vollum Institute

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Two new complementary DNAs overlapping cDNA clones that encode the G-protein coupled metabotropic glutamate receptor mGluRT from rat brain have been isolated and sequenced in their entirety. These new clones represent mRNA with 3' untranslated regions ∼2.5 kilobases longer than the previously isolated cDNA clones. These results indicate that the previously observed two size classes of ∼4 kb and ∼7 kb which hybridize to sequences that encode this receptor use different polyadenylation signals and differ in the extent of their 3' untranslated sequence. There is a striking asymmetry in the distribution of a sequence involved in mRNA instability between the two mRNA species.

Original language	English (US)
Pages (from-to)	53-57
Number of pages	5
Journal	Mitochondrial DNA
Volume	4
Issue number	1
DOIs	https://doi.org/10.3109/10425179309015623
State	Published - 1993

Keywords

CDNA
MRNA
Metabotropic glutamate receptor
Polyadenylation

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.3109/10425179309015623

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@article{b8f193c89e5246d2a5829050a00ca271,

title = "Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor",

abstract = "Two new complementary DNAs overlapping cDNA clones that encode the G-protein coupled metabotropic glutamate receptor mGluRT from rat brain have been isolated and sequenced in their entirety. These new clones represent mRNA with 3' untranslated regions ∼2.5 kilobases longer than the previously isolated cDNA clones. These results indicate that the previously observed two size classes of ∼4 kb and ∼7 kb which hybridize to sequences that encode this receptor use different polyadenylation signals and differ in the extent of their 3' untranslated sequence. There is a striking asymmetry in the distribution of a sequence involved in mRNA instability between the two mRNA species.",

keywords = "CDNA, MRNA, Metabotropic glutamate receptor, Polyadenylation",

author = "O'hara, {P. J.} and Gilbert, {T. L.} and Haldeman, {B. A.} and Houamed, {K. M.} and Kuijper, {J. L.} and Hagen, {F. S.} and W. Almers and E. Mulvihill",

note = "Funding Information: The authors thank Dr Terry Snutch for the kind gift of a cDNA library, Will Lint, Erica Vanaja and Vicki DeLoff for oligonucleotides, Paul Sheppard and Domenick Venezia for computer support and Frank Grant for discussions. Supported in part by NIH grant AR 17803 to W.A.",

year = "1993",

doi = "10.3109/10425179309015623",

language = "English (US)",

volume = "4",

pages = "53--57",

journal = "DNA Sequence - Journal of DNA Sequencing and Mapping",

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TY - JOUR

T1 - Different sites of polyadenylation in mRNAs encoding a rat metabotropic glutamate receptor

AU - O'hara, P. J.

AU - Gilbert, T. L.

AU - Haldeman, B. A.

AU - Houamed, K. M.

AU - Kuijper, J. L.

AU - Hagen, F. S.

AU - Almers, W.

AU - Mulvihill, E.

N1 - Funding Information: The authors thank Dr Terry Snutch for the kind gift of a cDNA library, Will Lint, Erica Vanaja and Vicki DeLoff for oligonucleotides, Paul Sheppard and Domenick Venezia for computer support and Frank Grant for discussions. Supported in part by NIH grant AR 17803 to W.A.

PY - 1993

Y1 - 1993

N2 - Two new complementary DNAs overlapping cDNA clones that encode the G-protein coupled metabotropic glutamate receptor mGluRT from rat brain have been isolated and sequenced in their entirety. These new clones represent mRNA with 3' untranslated regions ∼2.5 kilobases longer than the previously isolated cDNA clones. These results indicate that the previously observed two size classes of ∼4 kb and ∼7 kb which hybridize to sequences that encode this receptor use different polyadenylation signals and differ in the extent of their 3' untranslated sequence. There is a striking asymmetry in the distribution of a sequence involved in mRNA instability between the two mRNA species.

AB - Two new complementary DNAs overlapping cDNA clones that encode the G-protein coupled metabotropic glutamate receptor mGluRT from rat brain have been isolated and sequenced in their entirety. These new clones represent mRNA with 3' untranslated regions ∼2.5 kilobases longer than the previously isolated cDNA clones. These results indicate that the previously observed two size classes of ∼4 kb and ∼7 kb which hybridize to sequences that encode this receptor use different polyadenylation signals and differ in the extent of their 3' untranslated sequence. There is a striking asymmetry in the distribution of a sequence involved in mRNA instability between the two mRNA species.

KW - CDNA

KW - MRNA

KW - Metabotropic glutamate receptor

KW - Polyadenylation

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JO - DNA Sequence - Journal of DNA Sequencing and Mapping

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