Different outcomes in urethral reconstruction using elastin and collagen patches and conduits in rabbits

Hua Xie, Carmen E. Campbell, Brian S. Shaffer, Kenton Gregory

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objectives: To study the feasibility of urethral reconstruction with two urethroplasty techniques using an elastin and collagen heterograft in rabbits. Materials and Methods: Fifty-two male rabbits were studied. Two types of injury, (1) a 1.5 × 0.6 cm2 semicircumferential defect; (2) a 1.5 cm segmental defect of the penile urethra, were created and repaired using size-matched elastin and collagen patches or tubed conduits. Urethral repair by primary closure for the type 1 injury and a tubularized autologous bladder mucosal graft for the type 2 injury served as controls. At 3 months, urethral diameter was measured with retrograde urethrography. The animals were then euthanized for histological examination. Results: The postoperative complication rate was significantly higher in the urethral reconstructions using tubed collagen (83%) and elastin (50%) grafts compared to the patch onlay grafts (p = 0.001 for collagen and p = 0.01 for elastin) and tubularized ABM (10%, p = 0.003 and 0.05, respectively). At the type 2 injury site, a dense circumferential fibrosis developed after all repairs. Only minimal ventral fibrosis presented in the type 1 injury repair. The intensity of chronic inflammation and fibrosis was greatest when collagen was used for the urethral repair. In the elastin urethral repairs the urethral diameter decreased significantly for the tubed repair compared to the patch onlay (p = 0.02). Conclusion: Urethral injury repair using elastin and collagen biomaterials is feasible in the rabbit model. The results of onlay urethroplasty using the elastin and collagen patches are significantly superior to those using the elastin and collagen tubed conduits.

Original languageEnglish (US)
Pages (from-to)269-273
Number of pages5
JournalJournal of Biomedical Materials Research - Part B Applied Biomaterials
Volume81
Issue number1
DOIs
StatePublished - Apr 2007

Fingerprint

Elastin
Collagen
Repair
Grafts
Defects
Biocompatible Materials
Heterografts
Biomaterials
Animals

Keywords

  • Animal study
  • Biomaterial
  • Repair
  • Urethra
  • Urethroplasty

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials

Cite this

Different outcomes in urethral reconstruction using elastin and collagen patches and conduits in rabbits. / Xie, Hua; Campbell, Carmen E.; Shaffer, Brian S.; Gregory, Kenton.

In: Journal of Biomedical Materials Research - Part B Applied Biomaterials, Vol. 81, No. 1, 04.2007, p. 269-273.

Research output: Contribution to journalArticle

@article{c9699a0b02084bed87ac48d6c917a6a9,
title = "Different outcomes in urethral reconstruction using elastin and collagen patches and conduits in rabbits",
abstract = "Objectives: To study the feasibility of urethral reconstruction with two urethroplasty techniques using an elastin and collagen heterograft in rabbits. Materials and Methods: Fifty-two male rabbits were studied. Two types of injury, (1) a 1.5 × 0.6 cm2 semicircumferential defect; (2) a 1.5 cm segmental defect of the penile urethra, were created and repaired using size-matched elastin and collagen patches or tubed conduits. Urethral repair by primary closure for the type 1 injury and a tubularized autologous bladder mucosal graft for the type 2 injury served as controls. At 3 months, urethral diameter was measured with retrograde urethrography. The animals were then euthanized for histological examination. Results: The postoperative complication rate was significantly higher in the urethral reconstructions using tubed collagen (83{\%}) and elastin (50{\%}) grafts compared to the patch onlay grafts (p = 0.001 for collagen and p = 0.01 for elastin) and tubularized ABM (10{\%}, p = 0.003 and 0.05, respectively). At the type 2 injury site, a dense circumferential fibrosis developed after all repairs. Only minimal ventral fibrosis presented in the type 1 injury repair. The intensity of chronic inflammation and fibrosis was greatest when collagen was used for the urethral repair. In the elastin urethral repairs the urethral diameter decreased significantly for the tubed repair compared to the patch onlay (p = 0.02). Conclusion: Urethral injury repair using elastin and collagen biomaterials is feasible in the rabbit model. The results of onlay urethroplasty using the elastin and collagen patches are significantly superior to those using the elastin and collagen tubed conduits.",
keywords = "Animal study, Biomaterial, Repair, Urethra, Urethroplasty",
author = "Hua Xie and Campbell, {Carmen E.} and Shaffer, {Brian S.} and Kenton Gregory",
year = "2007",
month = "4",
doi = "10.1002/jbm.b.30662",
language = "English (US)",
volume = "81",
pages = "269--273",
journal = "Journal of Biomedical Materials Research - Part B Applied Biomaterials",
issn = "1552-4973",
publisher = "Heterocorporation",
number = "1",

}

TY - JOUR

T1 - Different outcomes in urethral reconstruction using elastin and collagen patches and conduits in rabbits

AU - Xie, Hua

AU - Campbell, Carmen E.

AU - Shaffer, Brian S.

AU - Gregory, Kenton

PY - 2007/4

Y1 - 2007/4

N2 - Objectives: To study the feasibility of urethral reconstruction with two urethroplasty techniques using an elastin and collagen heterograft in rabbits. Materials and Methods: Fifty-two male rabbits were studied. Two types of injury, (1) a 1.5 × 0.6 cm2 semicircumferential defect; (2) a 1.5 cm segmental defect of the penile urethra, were created and repaired using size-matched elastin and collagen patches or tubed conduits. Urethral repair by primary closure for the type 1 injury and a tubularized autologous bladder mucosal graft for the type 2 injury served as controls. At 3 months, urethral diameter was measured with retrograde urethrography. The animals were then euthanized for histological examination. Results: The postoperative complication rate was significantly higher in the urethral reconstructions using tubed collagen (83%) and elastin (50%) grafts compared to the patch onlay grafts (p = 0.001 for collagen and p = 0.01 for elastin) and tubularized ABM (10%, p = 0.003 and 0.05, respectively). At the type 2 injury site, a dense circumferential fibrosis developed after all repairs. Only minimal ventral fibrosis presented in the type 1 injury repair. The intensity of chronic inflammation and fibrosis was greatest when collagen was used for the urethral repair. In the elastin urethral repairs the urethral diameter decreased significantly for the tubed repair compared to the patch onlay (p = 0.02). Conclusion: Urethral injury repair using elastin and collagen biomaterials is feasible in the rabbit model. The results of onlay urethroplasty using the elastin and collagen patches are significantly superior to those using the elastin and collagen tubed conduits.

AB - Objectives: To study the feasibility of urethral reconstruction with two urethroplasty techniques using an elastin and collagen heterograft in rabbits. Materials and Methods: Fifty-two male rabbits were studied. Two types of injury, (1) a 1.5 × 0.6 cm2 semicircumferential defect; (2) a 1.5 cm segmental defect of the penile urethra, were created and repaired using size-matched elastin and collagen patches or tubed conduits. Urethral repair by primary closure for the type 1 injury and a tubularized autologous bladder mucosal graft for the type 2 injury served as controls. At 3 months, urethral diameter was measured with retrograde urethrography. The animals were then euthanized for histological examination. Results: The postoperative complication rate was significantly higher in the urethral reconstructions using tubed collagen (83%) and elastin (50%) grafts compared to the patch onlay grafts (p = 0.001 for collagen and p = 0.01 for elastin) and tubularized ABM (10%, p = 0.003 and 0.05, respectively). At the type 2 injury site, a dense circumferential fibrosis developed after all repairs. Only minimal ventral fibrosis presented in the type 1 injury repair. The intensity of chronic inflammation and fibrosis was greatest when collagen was used for the urethral repair. In the elastin urethral repairs the urethral diameter decreased significantly for the tubed repair compared to the patch onlay (p = 0.02). Conclusion: Urethral injury repair using elastin and collagen biomaterials is feasible in the rabbit model. The results of onlay urethroplasty using the elastin and collagen patches are significantly superior to those using the elastin and collagen tubed conduits.

KW - Animal study

KW - Biomaterial

KW - Repair

KW - Urethra

KW - Urethroplasty

UR - http://www.scopus.com/inward/record.url?scp=33947648387&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33947648387&partnerID=8YFLogxK

U2 - 10.1002/jbm.b.30662

DO - 10.1002/jbm.b.30662

M3 - Article

C2 - 16969825

AN - SCOPUS:33947648387

VL - 81

SP - 269

EP - 273

JO - Journal of Biomedical Materials Research - Part B Applied Biomaterials

JF - Journal of Biomedical Materials Research - Part B Applied Biomaterials

SN - 1552-4973

IS - 1

ER -