Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues

Takeshi Nakajima, Chiho Fukiage, Mitsuyoshi Azuma, Hong Ma, Thomas (Tom) Shearer

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The purpose of the present investigation was to compare the expression of ubiquitous and tissue-specific calpains in ocular tissues from the Macaca fascicularis monkey. Calpain isoforms in retina and corneal epithelium from adult M. fascicularis monkeys were characterized by RT-PCR, cDNA cloning and sequencing. Calpain isoform activities in ocular tissues were investigated by fractionation on DEAE-HPLC, immunoblotting, and casein zymography. Capn3 splice variants in the ocular tissues from rat, rabbit and monkey were compared after RT-PCR. RT-PCR analysis revealed that numerous splice variants of Capn3 were expressed in the epithelium from monkey cornea. The variants contained deletions or insertions in or around the IS1, IS2, and NS regions. The cDNAs for Capn3 variants were highly conserved, yet the expression patterns of the Capn3 isoforms were widely different among the mammalian species. In contrast, the expression patterns of ubiquitous calpains in ocular tissues were conserved among the mammalian species, and similarities between monkey and human cDNAs for Capn1 (μ-calpain) and Capn2 (m-calpain) were 98 and 99%, respectively. These results suggested that differences in expression patterns of Capn3 variants might be related to the function of each variant in a particular tissue or species.

Original languageEnglish (US)
Pages (from-to)55-64
Number of pages10
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1519
Issue number1-2
DOIs
StatePublished - May 28 2001

Fingerprint

Calpain
Haplorhini
Tissue
Protein Isoforms
Macaca fascicularis
Complementary DNA
Polymerase Chain Reaction
Corneal Epithelium
Cloning
Fractionation
Caseins
Immunoblotting
Cornea
Retina
Rats
Organism Cloning
Epithelium
High Pressure Liquid Chromatography
Rabbits

Keywords

  • Calpain
  • Calpain 3 isoform
  • Monkey
  • Proteinase
  • Rat

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Different expression patterns for ubiquitous calpains and Capn3 splice variants in monkey ocular tissues. / Nakajima, Takeshi; Fukiage, Chiho; Azuma, Mitsuyoshi; Ma, Hong; Shearer, Thomas (Tom).

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1519, No. 1-2, 28.05.2001, p. 55-64.

Research output: Contribution to journalArticle

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