Different Chinese hamster cell lines express a G1 period for different reasons

Robert (Mike) Liskay, Katherine E. Leonard, David M. Prescott

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Previous studies from our laboratory have shown that the absence of G1 (G1- condition) in two lines of Chinese hamster cells is dominant over the presence of G1 (G1+ condition) in a variety of intraspecific cell hybrids. G1+ mutants or variants can be isolated from G1- cells following mutagenesis and selection. These G1+ mutants fall into multiple complementation groups based on their abilities to form G1- cell hybrids with one another. This is evidence that different mutants have G1 intervals for different reasons, possibly as the result of deficiencies in functions necessary for G1- cell cycles. In this report we have used cell hybrid analysis to ask whether cells of different, naturally occurring G1+ lines of Chinese hamster are able to complement to produce G1- hybrids. We have found three complementation groups among the four G1+ cell lines examined. Therefore, these lines define three different reasons or bases for the existence of a G1 interval. These results lead us to suggest that multiple requirements must be met for these cells to start the S period, but that failure to fulfill only a single and different requirement is responsible for the presence of a G1 interval in any given cell line.

Original languageEnglish (US)
Pages (from-to)615-623
Number of pages9
JournalSomatic Cell Genetics
Volume5
Issue number5
DOIs
StatePublished - Sep 1979
Externally publishedYes

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Cricetulus
Hybrid Cells
Cell Line
S Phase
Mutagenesis
Cell Cycle

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Different Chinese hamster cell lines express a G1 period for different reasons. / Liskay, Robert (Mike); Leonard, Katherine E.; Prescott, David M.

In: Somatic Cell Genetics, Vol. 5, No. 5, 09.1979, p. 615-623.

Research output: Contribution to journalArticle

Liskay, Robert (Mike) ; Leonard, Katherine E. ; Prescott, David M. / Different Chinese hamster cell lines express a G1 period for different reasons. In: Somatic Cell Genetics. 1979 ; Vol. 5, No. 5. pp. 615-623.
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