Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice

Paul G. Kratzer, Verne M. Chapman, Hovey Lambert, Ronald E. Evans, Robert (Mike) Liskay

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

We have examined the role of DNA modification in X chromosome inactivation of fetal tissues of the mouse using DNA-mediated gene transfer for the gene hypoxanthine phosphoribosyltransferase (HPRT). Two types of tissues have been examined with respect to randomness of inactivation in 14-day mouse conceptuses: 1) fetal tissue, which under-goes random inactivation of either the maternal or paternal X; and 2) yolk sac endoderm tissue, an extraembryonic membrane, which normally undergoes nonrandom inactivation of the paternal X. Exploiting an electrophoretic variant of HPRT as a means to mark the active and inactive HPRT alleles we provide evidence that: 1) inactive X DNA of the fetus at 14 days behaves like that of both adult tissue and cell lines in that the inactive X DNA is not efficient in gene transfer; and 2) in contrast, inactive X DNA from yolk sac endoderm is functional in gene transfer. Thus, despite the similarity in single active X chromosome expression in yolk sac endoderm and somatic tissues, there appears to be a difference at the level of DNA modification between these two tissues.

Original languageEnglish (US)
Pages (from-to)37-42
Number of pages6
JournalCell
Volume33
Issue number1
DOIs
StatePublished - 1983
Externally publishedYes

Fingerprint

X Chromosome
Chromosomes
Fetus
Tissue
Hypoxanthine Phosphoribosyltransferase
Yolk Sac
Endoderm
Gene transfer
DNA
X Chromosome Inactivation
Genes
Extraembryonic Membranes
Alleles
Mothers
Cell Line
Cells
Membranes

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Kratzer, P. G., Chapman, V. M., Lambert, H., Evans, R. E., & Liskay, R. M. (1983). Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice. Cell, 33(1), 37-42. https://doi.org/10.1016/0092-8674(83)90332-X

Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice. / Kratzer, Paul G.; Chapman, Verne M.; Lambert, Hovey; Evans, Ronald E.; Liskay, Robert (Mike).

In: Cell, Vol. 33, No. 1, 1983, p. 37-42.

Research output: Contribution to journalArticle

Kratzer, PG, Chapman, VM, Lambert, H, Evans, RE & Liskay, RM 1983, 'Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice', Cell, vol. 33, no. 1, pp. 37-42. https://doi.org/10.1016/0092-8674(83)90332-X
Kratzer, Paul G. ; Chapman, Verne M. ; Lambert, Hovey ; Evans, Ronald E. ; Liskay, Robert (Mike). / Differences in the DNA of the inactive X chromosomes of fetal and extraembryonic tissues of mice. In: Cell. 1983 ; Vol. 33, No. 1. pp. 37-42.
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