@inproceedings{8fe8995c4f644e1685a2953600fb9d51,
title = "Dielectrophoretic (DEP) isolation and detection of cancer related cell Free DNA, RNA and exosome biomarkers from blood and plasma (Abstract # 600)",
abstract = "The analysis of cell free (cf) DNA and cf-RNA nanoparticulate biomarkers continues to become more widely used for cancer diagnostics and management. We have now demonstrated a dielectrophoretic (DEP) based approach that allows cf-DNA biomarkers from chronic lymphocytic leukemia (CLL) patients to be isolated in about 10 minutes directly from a small volume (25ul) of patient whole blood and plasma. Cf- DNA isolated from fifteen CLL patient blood samples and eleven CLL patient plasma samples were PCR amplified to identify the VHL genotype and then sequenced. The DNA sequencing results for cf-DNA isolated by DEP were compared to two gold standard methods for CLL analysis. In further work, cf-RNA for ROR1 has also been isolated from CLL samples, and RNA containing exosomes excreted by glioblastoma cells have been isolated by DEP.",
keywords = "Biomarkers, Cancer, Cell free DNA, Cell free RNA, DEP",
author = "Heller, {Michael J.} and Jennifer Marciniak and Stuart Ibsen and Elaine Skowronski and Avery Sonnenberg",
note = "Publisher Copyright: Copyright 2015 by TechConnect. All rights reserved.; 10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference ; Conference date: 14-06-2015 Through 17-06-2015",
year = "2015",
language = "English (US)",
series = "NSTI: Advanced Materials - TechConnect Briefs 2015",
publisher = "Taylor and Francis Inc.",
pages = "71--74",
editor = "Matthew Laudon and Bart Romanowicz",
booktitle = "NSTI",
}