Dielectrophoretic (DEP) isolation and detection of cancer related cell Free DNA, RNA and exosome biomarkers from blood and plasma (Abstract # 600)

Michael (Mike) Heller, Jennifer Marciniak, Stuart Ibsen, Elaine Skowronski, Avery Sonnenberg

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The analysis of cell free (cf) DNA and cf-RNA nanoparticulate biomarkers continues to become more widely used for cancer diagnostics and management. We have now demonstrated a dielectrophoretic (DEP) based approach that allows cf-DNA biomarkers from chronic lymphocytic leukemia (CLL) patients to be isolated in about 10 minutes directly from a small volume (25ul) of patient whole blood and plasma. Cf- DNA isolated from fifteen CLL patient blood samples and eleven CLL patient plasma samples were PCR amplified to identify the VHL genotype and then sequenced. The DNA sequencing results for cf-DNA isolated by DEP were compared to two gold standard methods for CLL analysis. In further work, cf-RNA for ROR1 has also been isolated from CLL samples, and RNA containing exosomes excreted by glioblastoma cells have been isolated by DEP.

Original languageEnglish (US)
Title of host publicationNSTI
Subtitle of host publicationBiotech, Biomaterials and Biomedical - TechConnect Briefs 2015
EditorsMatthew Laudon, Bart Romanowicz
PublisherTaylor and Francis Inc.
Pages71-74
Number of pages4
ISBN (Electronic)9781498747295
StatePublished - Jan 1 2015
Externally publishedYes
Event10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference - Washington, United States
Duration: Jun 14 2015Jun 17 2015

Publication series

NameNSTI: Advanced Materials - TechConnect Briefs 2015
Volume3

Conference

Conference10th Annual TechConnect World Innovation Conference and Expo, Held Jointly with the 18th Annual Nanotech Conference and Expo, and the 2015 National SBIR/STTR Conference
CountryUnited States
CityWashington
Period6/14/156/17/15

Keywords

  • Biomarkers
  • Cancer
  • Cell free DNA
  • Cell free RNA
  • DEP

ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Fluid Flow and Transfer Processes
  • Biotechnology
  • Fuel Technology

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  • Cite this

    Heller, M. M., Marciniak, J., Ibsen, S., Skowronski, E., & Sonnenberg, A. (2015). Dielectrophoretic (DEP) isolation and detection of cancer related cell Free DNA, RNA and exosome biomarkers from blood and plasma (Abstract # 600). In M. Laudon, & B. Romanowicz (Eds.), NSTI: Biotech, Biomaterials and Biomedical - TechConnect Briefs 2015 (pp. 71-74). (NSTI: Advanced Materials - TechConnect Briefs 2015; Vol. 3). Taylor and Francis Inc..