Dicentric chromosome frequency analysis using slit‐scan flow cytometry

Joe N. Lucas, James C. Mullikin, Joe W. Gray

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Slit‐scan flow cytometry (SSFCM) was used to quantify the frequency of dicentric chromosomes in human lymphoblastoid cells following γ irradiation. In this study, cultured human cells were irradiated with 0, 0.25, 0.5, 1.0, and 2.0 Gy of 0.66 MeV γ‐rays, cultured for an additional 11 h, and treated for 5 h with colcemid. Chromosomes were then isolated, stained with propidium iodide, and analyzed using SSFCM for total fluorescence and slit‐scan profile. The frequency of chromosomes having DNA contents greater than once and less than twice the DNA content of the number 1 chromosome and producing trimodal profiles was determined at each dose. This frequency was used as an estimate of the relative dicentric chromosome frequency at that dose. The estimated dicentric chromosome frequency per cell, f(D), increased with dose, D, in a linear‐quadratic manner according to the relation (Formula Presented.)

Original languageEnglish (US)
Pages (from-to)316-322
Number of pages7
JournalCytometry
Volume12
Issue number4
DOIs
StatePublished - 1991

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Keywords

  • Cs
  • Radiation
  • biological dosimetry
  • dose response
  • flow karyotyping
  • slit‐scan

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Biophysics
  • Hematology
  • Endocrinology
  • Cell Biology

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