Dicentric chromosome frequency analysis using slit-scan flow cytometry

J. N. Lucas, J. C. Mullikin, Joe Gray

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Slit-scan flow cytometry (SSFCM) was used to quantify the frequency of dicentric chromosomes in human lymphoblastoid cells following γ irradiation. In this study, cultured human cells were irradiated with 0, 0.25, 0.5, 1.0, and 2.0 Gy of 0.66 MeV γ-rays, cultured for an additional 11 h, and treated for 5 h with colcemid. Chromosomes were then isolated, stained with propidium iodide, and analyzed using SSFCM for total fluorescence and slit-scan profile. The frequency of chromosomes having DNA contents greater than once and less than twice the DNA content of the number 1 chromosome and producing trimodal profiles was determined at each dose. This frequency was used as an estimate of the relative dicentric chromosome frequency at that dose. The estimated dicentric chromosome frequency per cell, f(D), increased with dose, D, in a linear-quadratic manner according to the relation; f(D) = 4.52 x 10-5 + 5.72 x 10-5 D + 1.19 x 10-4 D2.

Original languageEnglish (US)
Pages (from-to)316-322
Number of pages7
JournalCytometry
Volume12
Issue number4
StatePublished - 1991
Externally publishedYes

Fingerprint

Flow Cytometry
Chromosomes
Demecolcine
Propidium
Chromosomes, Human, Pair 1
DNA
Human Chromosomes
Cultured Cells
Fluorescence

Keywords

  • Cs
  • biological dosimetry
  • dose response
  • flow karyotyping
  • radiation
  • slit-scan

ASJC Scopus subject areas

  • Hematology
  • Cell Biology
  • Pathology and Forensic Medicine
  • Biophysics
  • Endocrinology

Cite this

Dicentric chromosome frequency analysis using slit-scan flow cytometry. / Lucas, J. N.; Mullikin, J. C.; Gray, Joe.

In: Cytometry, Vol. 12, No. 4, 1991, p. 316-322.

Research output: Contribution to journalArticle

Lucas, JN, Mullikin, JC & Gray, J 1991, 'Dicentric chromosome frequency analysis using slit-scan flow cytometry', Cytometry, vol. 12, no. 4, pp. 316-322.
Lucas, J. N. ; Mullikin, J. C. ; Gray, Joe. / Dicentric chromosome frequency analysis using slit-scan flow cytometry. In: Cytometry. 1991 ; Vol. 12, No. 4. pp. 316-322.
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