Diaphanous-related formins bridge Rho GTPase and Src tyrosine kinase signaling

Tomoko Tominaga, Erik Sahai, Pierre Chardin, Frank McCormick, Sara A. Courtneidge, Arthur S. Alberts

Research output: Contribution to journalArticle

311 Scopus citations

Abstract

We have examined the role of the mouse Diaphanous-related formin (DRF) Rho GTPase binding proteins, mDia1 and mDia2, in cell regulation. The DRFs are required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor (SRF). 'Activated' mDia1 and mDia2 variants, lacking their GTPase binding domains, cooperated with Rho-kinase or ROCK to form stress fibers but independently activated SRF. Src tyrosine kinase associated and co-localized with the DRFs in endosomes and in mid-bodies of dividing cells. Inhibition of Src also blocked cytokinesis, SRF induction by activated DRFs, and cooperative stress fiber formation with active ROCK. Our results show that the DRF proteins couple Rho and Src during signaling and the regulation of actin dynamics.

Original languageEnglish (US)
Pages (from-to)13-25
Number of pages13
JournalMolecular Cell
Volume5
Issue number1
DOIs
StatePublished - Jan 2000

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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