Dialysable leukocyte extracts (transfer factor) augment nonspecifically keyhole limpet haemocyanin and horseshoe crab haemocyanin skin reactivity in unimmunized human recipients

R. G I Ashorn, Arthur Vandenbark, K. M. Acott, K. J E Krohn

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Dialysable leukocyte extracts (DLE) may induce marked changes in the immune expression of human recipients. It is unclear whether the conversion of skin reactivity by DLE is due to a donor-related specific transfer factor or to an antigen nonspecific augmenting factor with enhances a preexisting low-level response in DLE recipients. In this study, DLE from immunized and unimmunized human and calf donors or saline was administered to 88 medical students. The recipient population demonstrated minimal background responses to the test antigens keyhole limpet haemocyanin (KLH) and horseshoe crab haemocyanin (HCH). The results indicate that the DLE preparations from both immunized and unimmunized donors significantly stimulated skin reactivity but not in vitro responses to both KLH and HCH in the recipient population. The results suggest that these DLE preparations contain an immunologically nonspecific augmentor, which stimulates a preexisting low-level response in the unimmunized population to become a clearly observable skin reaction.

Original languageEnglish (US)
Pages (from-to)161-167
Number of pages7
JournalScandinavian Journal of Immunology
Volume23
Issue number2
StatePublished - 1986

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Transfer Factor
Horseshoe Crabs
Hemocyanin
Leukocytes
Skin
Tissue Donors
Population
Antigens
Medical Students
keyhole-limpet hemocyanin

ASJC Scopus subject areas

  • Immunology

Cite this

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title = "Dialysable leukocyte extracts (transfer factor) augment nonspecifically keyhole limpet haemocyanin and horseshoe crab haemocyanin skin reactivity in unimmunized human recipients",
abstract = "Dialysable leukocyte extracts (DLE) may induce marked changes in the immune expression of human recipients. It is unclear whether the conversion of skin reactivity by DLE is due to a donor-related specific transfer factor or to an antigen nonspecific augmenting factor with enhances a preexisting low-level response in DLE recipients. In this study, DLE from immunized and unimmunized human and calf donors or saline was administered to 88 medical students. The recipient population demonstrated minimal background responses to the test antigens keyhole limpet haemocyanin (KLH) and horseshoe crab haemocyanin (HCH). The results indicate that the DLE preparations from both immunized and unimmunized donors significantly stimulated skin reactivity but not in vitro responses to both KLH and HCH in the recipient population. The results suggest that these DLE preparations contain an immunologically nonspecific augmentor, which stimulates a preexisting low-level response in the unimmunized population to become a clearly observable skin reaction.",
author = "Ashorn, {R. G I} and Arthur Vandenbark and Acott, {K. M.} and Krohn, {K. J E}",
year = "1986",
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T1 - Dialysable leukocyte extracts (transfer factor) augment nonspecifically keyhole limpet haemocyanin and horseshoe crab haemocyanin skin reactivity in unimmunized human recipients

AU - Ashorn, R. G I

AU - Vandenbark, Arthur

AU - Acott, K. M.

AU - Krohn, K. J E

PY - 1986

Y1 - 1986

N2 - Dialysable leukocyte extracts (DLE) may induce marked changes in the immune expression of human recipients. It is unclear whether the conversion of skin reactivity by DLE is due to a donor-related specific transfer factor or to an antigen nonspecific augmenting factor with enhances a preexisting low-level response in DLE recipients. In this study, DLE from immunized and unimmunized human and calf donors or saline was administered to 88 medical students. The recipient population demonstrated minimal background responses to the test antigens keyhole limpet haemocyanin (KLH) and horseshoe crab haemocyanin (HCH). The results indicate that the DLE preparations from both immunized and unimmunized donors significantly stimulated skin reactivity but not in vitro responses to both KLH and HCH in the recipient population. The results suggest that these DLE preparations contain an immunologically nonspecific augmentor, which stimulates a preexisting low-level response in the unimmunized population to become a clearly observable skin reaction.

AB - Dialysable leukocyte extracts (DLE) may induce marked changes in the immune expression of human recipients. It is unclear whether the conversion of skin reactivity by DLE is due to a donor-related specific transfer factor or to an antigen nonspecific augmenting factor with enhances a preexisting low-level response in DLE recipients. In this study, DLE from immunized and unimmunized human and calf donors or saline was administered to 88 medical students. The recipient population demonstrated minimal background responses to the test antigens keyhole limpet haemocyanin (KLH) and horseshoe crab haemocyanin (HCH). The results indicate that the DLE preparations from both immunized and unimmunized donors significantly stimulated skin reactivity but not in vitro responses to both KLH and HCH in the recipient population. The results suggest that these DLE preparations contain an immunologically nonspecific augmentor, which stimulates a preexisting low-level response in the unimmunized population to become a clearly observable skin reaction.

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