Diagnostic Values of Cerebrospinal Fluid T-Tau and Aβ42 using Meso Scale Discovery Assays for Alzheimer's Disease

Catherine Pan, Ané Korff, Douglas Galasko, Carmen Ginghina, Elaine Peskind, Ge Li, Joseph Quinn, Thomas J. Montine, Kevin Cain, Min Shi, Jing Zhang

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Background: Meso Scale Discovery (MSD) recently established electrochemiluminescence-based assays to measure cerebrospinal fluid (CSF) levels of total tau (t-tau) and amyloid-β 1-42 peptide (Aβ42) that can aid in the diagnosis of Alzheimer's disease (AD). The goal of this investigation is to independently evaluate this platform and establish cut-off values of these biomarkers for AD diagnosis. Objective: To validate the analytical and clinical performance of the MSD t-tau and Aβ42 kits and propose diagnostic cut-off values for the field. Methods: The analytical performance of the CSF t-tau and Aβ42 assays was determined, followed by assessment of diagnostic performance of CSF t-tau, Aβ42, and t-tau/Aβ42 in three clinically characterized cohorts. Results: Both MSD assays demonstrated consistent and stable analytical performance, as well as resistance to several important pre-analytic variables. Diagnostically, t-tau/Aβ42 performed the best. Conclusions: Our results independently confirm the analytical and clinical performance of the MSD CSF t-tau and Aβ42 assays. Based on a large, multi-center, clinically-diagnosed cohort, we propose for the first time candidate diagnostic cut-offs for MSD measured CSF t-tau, Aβ42, and t-tau/Aβ42. However, these values needs to be refined as more subjects are included and the assays are tested by other laboratories.

Original languageEnglish (US)
Pages (from-to)709-719
Number of pages11
JournalJournal of Alzheimer's Disease
Volume45
Issue number3
DOIs
StatePublished - Jan 1 2015

Keywords

  • Alzheimer's disease
  • Meso Scale Discovery
  • cerebrospinal fluid
  • tau

ASJC Scopus subject areas

  • Neuroscience(all)
  • Clinical Psychology
  • Geriatrics and Gerontology
  • Psychiatry and Mental health

Fingerprint Dive into the research topics of 'Diagnostic Values of Cerebrospinal Fluid T-Tau and Aβ<sub>42</sub> using Meso Scale Discovery Assays for Alzheimer's Disease'. Together they form a unique fingerprint.

  • Cite this

    Pan, C., Korff, A., Galasko, D., Ginghina, C., Peskind, E., Li, G., Quinn, J., Montine, T. J., Cain, K., Shi, M., & Zhang, J. (2015). Diagnostic Values of Cerebrospinal Fluid T-Tau and Aβ42 using Meso Scale Discovery Assays for Alzheimer's Disease. Journal of Alzheimer's Disease, 45(3), 709-719. https://doi.org/10.3233/JAD-143099