DH and JH usage in murine fetal liver mirrors that of human fetal liver

Robert Schelonka, Ewa Szymanska, Andre M. Vale, Yingxin Zhuang, G. Larry Gartland, Harry W. Schroeder

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

In mouse and human, the regulated development of antibody repertoire diversity during ontogeny proceeds in parallel with the development of the ability to generate antibodies to an array of specific antigens. Compared to adult, the human fetal antibody repertoire limits N addition and uses specifically positioned VDJ gene segments more frequently, including V6-1 the most DH-proximal VH, DQ52, the most JH-proximal DH, and JH2, which is DH-proximal. The murine fetal antibody repertoire also limits the incorporation of N nucleotides and uses its most DH proximal VH, VH81X, more frequently. To test whether DH and JH also follow the pattern observed in human, we used the scheme of Hardy to sort B lineage cells from BALB/c fetal and neonatal liver, RT-PCR cloned and sequenced V H7183-containing VDJCμ transcripts, and then assessed V H7183-DH-JH and complementary determining region 3 of the immunoglobulin heavy chain (CDR-H3) content in comparison to the previously studied adult BALB/c mouse repertoire. Due to the deficiency in N nucleotide addition, perinatal CDR-H3s manifested a distinct pattern of amino acid usage and predicted loop structures. As in the case of adult bone marrow, we observed a focusing of CDR-H3 length and CDR-H3 loop hydrophobicity, especially in the transition from the early to late pre-B cell stage, a developmental checkpoint associated with expression of the pre-B cell receptor. However, fetal liver usage of JH-proximal DHQ52 and D H-proximal JH2 was markedly greater than that of adult bone marrow. Thus, the early pattern of DH and JH usage in mouse feta liver mirrors that of human.

Original languageEnglish (US)
Pages (from-to)653-666
Number of pages14
JournalImmunogenetics
Volume62
Issue number10
DOIs
StatePublished - Oct 2010

Fingerprint

Antibodies
Liver
Pre-B Cell Receptors
Antibody Diversity
Nucleotides
Bone Marrow
Immunoglobulin Heavy Chains
Aptitude
B-Lymphoid Precursor Cells
Human Development
Hydrophobic and Hydrophilic Interactions
Antigens
Amino Acids
Polymerase Chain Reaction
Genes

Keywords

  • Adult mouse repertoire
  • CDR-H3
  • Fetal mouse repertoire

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Schelonka, R., Szymanska, E., Vale, A. M., Zhuang, Y., Gartland, G. L., & Schroeder, H. W. (2010). DH and JH usage in murine fetal liver mirrors that of human fetal liver. Immunogenetics, 62(10), 653-666. https://doi.org/10.1007/s00251-010-0469-5

DH and JH usage in murine fetal liver mirrors that of human fetal liver. / Schelonka, Robert; Szymanska, Ewa; Vale, Andre M.; Zhuang, Yingxin; Gartland, G. Larry; Schroeder, Harry W.

In: Immunogenetics, Vol. 62, No. 10, 10.2010, p. 653-666.

Research output: Contribution to journalArticle

Schelonka, R, Szymanska, E, Vale, AM, Zhuang, Y, Gartland, GL & Schroeder, HW 2010, 'DH and JH usage in murine fetal liver mirrors that of human fetal liver', Immunogenetics, vol. 62, no. 10, pp. 653-666. https://doi.org/10.1007/s00251-010-0469-5
Schelonka, Robert ; Szymanska, Ewa ; Vale, Andre M. ; Zhuang, Yingxin ; Gartland, G. Larry ; Schroeder, Harry W. / DH and JH usage in murine fetal liver mirrors that of human fetal liver. In: Immunogenetics. 2010 ; Vol. 62, No. 10. pp. 653-666.
@article{ff387f91b0664847ac55a2962356505c,
title = "DH and JH usage in murine fetal liver mirrors that of human fetal liver",
abstract = "In mouse and human, the regulated development of antibody repertoire diversity during ontogeny proceeds in parallel with the development of the ability to generate antibodies to an array of specific antigens. Compared to adult, the human fetal antibody repertoire limits N addition and uses specifically positioned VDJ gene segments more frequently, including V6-1 the most DH-proximal VH, DQ52, the most JH-proximal DH, and JH2, which is DH-proximal. The murine fetal antibody repertoire also limits the incorporation of N nucleotides and uses its most DH proximal VH, VH81X, more frequently. To test whether DH and JH also follow the pattern observed in human, we used the scheme of Hardy to sort B lineage cells from BALB/c fetal and neonatal liver, RT-PCR cloned and sequenced V H7183-containing VDJCμ transcripts, and then assessed V H7183-DH-JH and complementary determining region 3 of the immunoglobulin heavy chain (CDR-H3) content in comparison to the previously studied adult BALB/c mouse repertoire. Due to the deficiency in N nucleotide addition, perinatal CDR-H3s manifested a distinct pattern of amino acid usage and predicted loop structures. As in the case of adult bone marrow, we observed a focusing of CDR-H3 length and CDR-H3 loop hydrophobicity, especially in the transition from the early to late pre-B cell stage, a developmental checkpoint associated with expression of the pre-B cell receptor. However, fetal liver usage of JH-proximal DHQ52 and D H-proximal JH2 was markedly greater than that of adult bone marrow. Thus, the early pattern of DH and JH usage in mouse feta liver mirrors that of human.",
keywords = "Adult mouse repertoire, CDR-H3, Fetal mouse repertoire",
author = "Robert Schelonka and Ewa Szymanska and Vale, {Andre M.} and Yingxin Zhuang and Gartland, {G. Larry} and Schroeder, {Harry W.}",
year = "2010",
month = "10",
doi = "10.1007/s00251-010-0469-5",
language = "English (US)",
volume = "62",
pages = "653--666",
journal = "Immunogenetics",
issn = "0093-7711",
publisher = "Springer Verlag",
number = "10",

}

TY - JOUR

T1 - DH and JH usage in murine fetal liver mirrors that of human fetal liver

AU - Schelonka, Robert

AU - Szymanska, Ewa

AU - Vale, Andre M.

AU - Zhuang, Yingxin

AU - Gartland, G. Larry

AU - Schroeder, Harry W.

PY - 2010/10

Y1 - 2010/10

N2 - In mouse and human, the regulated development of antibody repertoire diversity during ontogeny proceeds in parallel with the development of the ability to generate antibodies to an array of specific antigens. Compared to adult, the human fetal antibody repertoire limits N addition and uses specifically positioned VDJ gene segments more frequently, including V6-1 the most DH-proximal VH, DQ52, the most JH-proximal DH, and JH2, which is DH-proximal. The murine fetal antibody repertoire also limits the incorporation of N nucleotides and uses its most DH proximal VH, VH81X, more frequently. To test whether DH and JH also follow the pattern observed in human, we used the scheme of Hardy to sort B lineage cells from BALB/c fetal and neonatal liver, RT-PCR cloned and sequenced V H7183-containing VDJCμ transcripts, and then assessed V H7183-DH-JH and complementary determining region 3 of the immunoglobulin heavy chain (CDR-H3) content in comparison to the previously studied adult BALB/c mouse repertoire. Due to the deficiency in N nucleotide addition, perinatal CDR-H3s manifested a distinct pattern of amino acid usage and predicted loop structures. As in the case of adult bone marrow, we observed a focusing of CDR-H3 length and CDR-H3 loop hydrophobicity, especially in the transition from the early to late pre-B cell stage, a developmental checkpoint associated with expression of the pre-B cell receptor. However, fetal liver usage of JH-proximal DHQ52 and D H-proximal JH2 was markedly greater than that of adult bone marrow. Thus, the early pattern of DH and JH usage in mouse feta liver mirrors that of human.

AB - In mouse and human, the regulated development of antibody repertoire diversity during ontogeny proceeds in parallel with the development of the ability to generate antibodies to an array of specific antigens. Compared to adult, the human fetal antibody repertoire limits N addition and uses specifically positioned VDJ gene segments more frequently, including V6-1 the most DH-proximal VH, DQ52, the most JH-proximal DH, and JH2, which is DH-proximal. The murine fetal antibody repertoire also limits the incorporation of N nucleotides and uses its most DH proximal VH, VH81X, more frequently. To test whether DH and JH also follow the pattern observed in human, we used the scheme of Hardy to sort B lineage cells from BALB/c fetal and neonatal liver, RT-PCR cloned and sequenced V H7183-containing VDJCμ transcripts, and then assessed V H7183-DH-JH and complementary determining region 3 of the immunoglobulin heavy chain (CDR-H3) content in comparison to the previously studied adult BALB/c mouse repertoire. Due to the deficiency in N nucleotide addition, perinatal CDR-H3s manifested a distinct pattern of amino acid usage and predicted loop structures. As in the case of adult bone marrow, we observed a focusing of CDR-H3 length and CDR-H3 loop hydrophobicity, especially in the transition from the early to late pre-B cell stage, a developmental checkpoint associated with expression of the pre-B cell receptor. However, fetal liver usage of JH-proximal DHQ52 and D H-proximal JH2 was markedly greater than that of adult bone marrow. Thus, the early pattern of DH and JH usage in mouse feta liver mirrors that of human.

KW - Adult mouse repertoire

KW - CDR-H3

KW - Fetal mouse repertoire

UR - http://www.scopus.com/inward/record.url?scp=77957571907&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957571907&partnerID=8YFLogxK

U2 - 10.1007/s00251-010-0469-5

DO - 10.1007/s00251-010-0469-5

M3 - Article

VL - 62

SP - 653

EP - 666

JO - Immunogenetics

JF - Immunogenetics

SN - 0093-7711

IS - 10

ER -