Dexamethasone reduces steady state insulin-like growth factor I messenger ribonucleic acid levels in rat neuronal and glial cells in primary culture

M. Adamo, H. Werner, W. Farnsworth, Charles Roberts, M. Raizada, D. LeRoith

Research output: Contribution to journalArticle

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Abstract

Insulin-like growth factor I (IGF-I) mRNA was demonstrated in primary cultures of neuronal and glial cells from rat brain. On Northern blots, a rat IGF-I cDNA probe hybridized to RNA species of 7.5, 1.7, and 0.8-1.2 kilobases in total and poly(A)+ RNA from both cell types. Solution hybridization/RNase protection assays were performed using an antisense riboprobe complementary to the 5'-untranslated region as well as part of the coding region of rat IGF-I mRNA. These studies indicated that two of the previously described three possible alternative 5'-untranslated splicing variants (classes A and C) were expressed in neuronal and glial cells, with class C transcripts predominating. Neuronal cells also possessed extremely low levels of class B transcripts. Treatment of neuronal cell cultures with the synthetic glucocorticoid dexamethasone reduced IGF-I mRNA levels by 60%. Glial cell IGF-I mRNA levels were reduced by dexamethasone by up to 40%. These results suggest that glucocorticoid-induced reductions in IGF-I production could occur at the level of transcription and may underlie some of the actions of glucocorticoids in causing growth retardation and inhibition of cell proliferation.

Original languageEnglish (US)
Pages (from-to)2565-2570
Number of pages6
JournalEndocrinology
Volume123
Issue number5
StatePublished - 1988
Externally publishedYes

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Insulin-Like Growth Factor I
Neuroglia
Dexamethasone
RNA
Messenger RNA
Glucocorticoids
5' Untranslated Regions
Ribonucleases
Northern Blotting
Complementary DNA
Cell Culture Techniques
Cell Proliferation
Brain
Growth

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Dexamethasone reduces steady state insulin-like growth factor I messenger ribonucleic acid levels in rat neuronal and glial cells in primary culture. / Adamo, M.; Werner, H.; Farnsworth, W.; Roberts, Charles; Raizada, M.; LeRoith, D.

In: Endocrinology, Vol. 123, No. 5, 1988, p. 2565-2570.

Research output: Contribution to journalArticle

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AU - LeRoith, D.

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