Abstract
Objective: To investigate the interaction of dexamethasone and interleukin-1β (IL-1β) on the expression of cytosolic phospholipase A2 (cPLA2), the enzyme catalyzing the first reaction in the formation of prostaglandins, in cultured primary human amnion fibroblasts. Design and methods: Human amnion fibroblasts were prepared from fetal amnion collected at term and were treated with dexamethasone with or without interleukin-1β for 24 h. Prostaglandin E2 (PGE2) output and cPLA2 expression in cultured amnion fibroblasts were measured with radioimmunoassay, quantitative real-time polymerase chain reaction, Western blotting and cPLA2 promoter-driven luciferase reporter gene activity. Results: Both dexamethasone and IL-1β caused a significant increase in prostaglandin E2 output, cPLA2 mRNA and protein expression in cultured human amnion fibroblasts. Both dexamethasone and IL-1β stimulated cPLA2 promoter-driven luciferase reporter gene activity. There was no obvious antagonistic or synergistic effect of combined treatment of dexamethasone and IL-1β on PGE2 output, cPLA2 expression or cPLA2 promoter-driven luciferase reporter gene activity in cultured human amnion fibroblasts. Conclusion: The above findings suggest that paradoxically dexamethasone is a stimulator for both prostaglandin synthesis and cPLA2 expression in human amnion fibroblasts. The interaction between dexamethasone and IL-1β on prostaglandin synthesis and cPLA2 expression is neither synergistic nor conventionally antagonistic.
Original language | English (US) |
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Pages (from-to) | 164-170 |
Number of pages | 7 |
Journal | Placenta |
Volume | 27 |
Issue number | 2-3 |
DOIs | |
State | Published - Feb 2006 |
Externally published | Yes |
Keywords
- Amnion
- Glucocorticoids
- Parturition
- Proinflammatory cytokines
- Prostaglandins
ASJC Scopus subject areas
- Reproductive Medicine
- Obstetrics and Gynecology
- Developmental Biology