Developmental time course of acidic and basic fibroblast growth factors' expression in distinct cellular populations of the rat central nervous system

K. Kuzis, S. Reed, N. J. Cherry, William Woodward, F. P. Eckenstein

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) are expressed in high levels in adult central nervous system (CNS). We report the time course of developmental appearance and distribution of these factors and of two FGF receptors, FGFR-1 and FGFR-2, in the CNS of rats ranging in age from embryonic day 16 to adult. Immunohistochemical analysis showed that sensory neurons in the midbrain were the first cells to contain detectable aFGF immunoreactivity at embryonic day 18. The next cell group to contain aFGF were motor neurons, which were found to be aFGF-positive at the day of birth. A number of other subcortical neuronal populations were observed to contain aFGF immunoreactivity after postnatal day 7. Adult levels and distribution patterns of aFGF were reached in all CNS areas by postnatal day 28. Basic FGF immunoreactivity was observed at postnatal day 0 in neurons in the CA2 subfield of hippocampus. Astrocytes contained detectable bFGF immunoreactivity, starting at postnatal day 7. Adult levels and patterns of distribution of bFGF were reached in all CNS areas by postnatal day 28. These immunohistochemical observations were confirmed by using bioassay and Western blot techniques. FGFR-1 and FGFR 2 mRNA were expressed in significant levels in all CNS areas at all time points analyzed. The observation that aFGF and bFGF appear in specific and distinct cellular populations at relatively late developmental times suggests that these FGFs may be involved in specific mechanisms of CNS maturation, maintenance, and repair.

Original languageEnglish (US)
Pages (from-to)142-153
Number of pages12
JournalJournal of Comparative Neurology
Volume358
Issue number1
DOIs
StatePublished - 1995

Fingerprint

Fibroblast Growth Factor 1
Fibroblast Growth Factor 2
Central Nervous System
Population
Fibroblast Growth Factor Receptors
Motor Neurons
Sensory Receptor Cells
Mesencephalon
Astrocytes
Biological Assay
Hippocampus
Western Blotting
Maintenance
Parturition
Neurons
Messenger RNA

Keywords

  • astrocyte
  • heparin binding growth factor
  • injury
  • motoneuron
  • repair

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Developmental time course of acidic and basic fibroblast growth factors' expression in distinct cellular populations of the rat central nervous system. / Kuzis, K.; Reed, S.; Cherry, N. J.; Woodward, William; Eckenstein, F. P.

In: Journal of Comparative Neurology, Vol. 358, No. 1, 1995, p. 142-153.

Research output: Contribution to journalArticle

@article{737fabd1697a4074b2c202e685b59865,
title = "Developmental time course of acidic and basic fibroblast growth factors' expression in distinct cellular populations of the rat central nervous system",
abstract = "Acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) are expressed in high levels in adult central nervous system (CNS). We report the time course of developmental appearance and distribution of these factors and of two FGF receptors, FGFR-1 and FGFR-2, in the CNS of rats ranging in age from embryonic day 16 to adult. Immunohistochemical analysis showed that sensory neurons in the midbrain were the first cells to contain detectable aFGF immunoreactivity at embryonic day 18. The next cell group to contain aFGF were motor neurons, which were found to be aFGF-positive at the day of birth. A number of other subcortical neuronal populations were observed to contain aFGF immunoreactivity after postnatal day 7. Adult levels and distribution patterns of aFGF were reached in all CNS areas by postnatal day 28. Basic FGF immunoreactivity was observed at postnatal day 0 in neurons in the CA2 subfield of hippocampus. Astrocytes contained detectable bFGF immunoreactivity, starting at postnatal day 7. Adult levels and patterns of distribution of bFGF were reached in all CNS areas by postnatal day 28. These immunohistochemical observations were confirmed by using bioassay and Western blot techniques. FGFR-1 and FGFR 2 mRNA were expressed in significant levels in all CNS areas at all time points analyzed. The observation that aFGF and bFGF appear in specific and distinct cellular populations at relatively late developmental times suggests that these FGFs may be involved in specific mechanisms of CNS maturation, maintenance, and repair.",
keywords = "astrocyte, heparin binding growth factor, injury, motoneuron, repair",
author = "K. Kuzis and S. Reed and Cherry, {N. J.} and William Woodward and Eckenstein, {F. P.}",
year = "1995",
doi = "10.1002/cne.903580109",
language = "English (US)",
volume = "358",
pages = "142--153",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "1",

}

TY - JOUR

T1 - Developmental time course of acidic and basic fibroblast growth factors' expression in distinct cellular populations of the rat central nervous system

AU - Kuzis, K.

AU - Reed, S.

AU - Cherry, N. J.

AU - Woodward, William

AU - Eckenstein, F. P.

PY - 1995

Y1 - 1995

N2 - Acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) are expressed in high levels in adult central nervous system (CNS). We report the time course of developmental appearance and distribution of these factors and of two FGF receptors, FGFR-1 and FGFR-2, in the CNS of rats ranging in age from embryonic day 16 to adult. Immunohistochemical analysis showed that sensory neurons in the midbrain were the first cells to contain detectable aFGF immunoreactivity at embryonic day 18. The next cell group to contain aFGF were motor neurons, which were found to be aFGF-positive at the day of birth. A number of other subcortical neuronal populations were observed to contain aFGF immunoreactivity after postnatal day 7. Adult levels and distribution patterns of aFGF were reached in all CNS areas by postnatal day 28. Basic FGF immunoreactivity was observed at postnatal day 0 in neurons in the CA2 subfield of hippocampus. Astrocytes contained detectable bFGF immunoreactivity, starting at postnatal day 7. Adult levels and patterns of distribution of bFGF were reached in all CNS areas by postnatal day 28. These immunohistochemical observations were confirmed by using bioassay and Western blot techniques. FGFR-1 and FGFR 2 mRNA were expressed in significant levels in all CNS areas at all time points analyzed. The observation that aFGF and bFGF appear in specific and distinct cellular populations at relatively late developmental times suggests that these FGFs may be involved in specific mechanisms of CNS maturation, maintenance, and repair.

AB - Acidic and basic fibroblast growth factors (aFGF and bFGF, respectively) are expressed in high levels in adult central nervous system (CNS). We report the time course of developmental appearance and distribution of these factors and of two FGF receptors, FGFR-1 and FGFR-2, in the CNS of rats ranging in age from embryonic day 16 to adult. Immunohistochemical analysis showed that sensory neurons in the midbrain were the first cells to contain detectable aFGF immunoreactivity at embryonic day 18. The next cell group to contain aFGF were motor neurons, which were found to be aFGF-positive at the day of birth. A number of other subcortical neuronal populations were observed to contain aFGF immunoreactivity after postnatal day 7. Adult levels and distribution patterns of aFGF were reached in all CNS areas by postnatal day 28. Basic FGF immunoreactivity was observed at postnatal day 0 in neurons in the CA2 subfield of hippocampus. Astrocytes contained detectable bFGF immunoreactivity, starting at postnatal day 7. Adult levels and patterns of distribution of bFGF were reached in all CNS areas by postnatal day 28. These immunohistochemical observations were confirmed by using bioassay and Western blot techniques. FGFR-1 and FGFR 2 mRNA were expressed in significant levels in all CNS areas at all time points analyzed. The observation that aFGF and bFGF appear in specific and distinct cellular populations at relatively late developmental times suggests that these FGFs may be involved in specific mechanisms of CNS maturation, maintenance, and repair.

KW - astrocyte

KW - heparin binding growth factor

KW - injury

KW - motoneuron

KW - repair

UR - http://www.scopus.com/inward/record.url?scp=0029032715&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029032715&partnerID=8YFLogxK

U2 - 10.1002/cne.903580109

DO - 10.1002/cne.903580109

M3 - Article

C2 - 7560275

AN - SCOPUS:0029032715

VL - 358

SP - 142

EP - 153

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 1

ER -