Developmental regulation of insulin-like growth factor-I-stimulated glucose transporter in rat brain astrocytes

Brian A. Masters, Haim Werner, Charles Roberts, Derek Leroith, Mohan K. Raizada

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Astrocytic glial cells from 1- and 21-day-old rat brains were established in primary culture to study the expression of insulin-like growth factor-I (IGF-I) receptors and IGF-I-stimulated glucose transporter (Glut-1). Astrocytes from both age groups expressed specific high affinity IGF-I receptors, whose relative affinities for IGF-I, IGF-II, and insulin were comparable. However, the total number of binding sites and IGF-I receptor mRNA levels were 148% and 240% higher in astrocytes from 21-day-old compared with 1-day-old brains. IGF-I caused a dose-dependent stimulation of [3H]2-deoxy-D-glucose ([3H]dGlc) uptake in astrocytes from 1-day-old brains. This was associated with increases in Glut-1 protein and mRNA levels. In contrast, astrocytes from 21-day-old brains exhibited a 58% decrease in the binding capacity and a 77% decrease in the steady state levels of Glut-1 protein and its mRNA. In addition, IGF-I failed to stimulate the Glut-1 system in these cells. This lack of IGF-I effect is not due to an alteration inherent to the Glut-1 system, since 12-O-tetradecanoyl-phorbol-13-acetate stimulated [3H]dGlc uptake and Glut-1 protein and its mRNA levels. These observations suggest that changes in basal and IGF-I-stimulated Glut-1 system in brain astrocytes may be developmentally regulated.

Original languageEnglish (US)
Pages (from-to)2548-2557
Number of pages10
JournalEndocrinology
Volume128
Issue number5
StatePublished - May 1991
Externally publishedYes

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Facilitative Glucose Transport Proteins
Insulin-Like Growth Factor I
Astrocytes
IGF Type 1 Receptor
Brain
Messenger RNA
Proteins
Insulin-Like Growth Factor II
Deoxyglucose
Tetradecanoylphorbol Acetate
Neuroglia
Age Groups
Binding Sites
Insulin

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Developmental regulation of insulin-like growth factor-I-stimulated glucose transporter in rat brain astrocytes. / Masters, Brian A.; Werner, Haim; Roberts, Charles; Leroith, Derek; Raizada, Mohan K.

In: Endocrinology, Vol. 128, No. 5, 05.1991, p. 2548-2557.

Research output: Contribution to journalArticle

Masters, BA, Werner, H, Roberts, C, Leroith, D & Raizada, MK 1991, 'Developmental regulation of insulin-like growth factor-I-stimulated glucose transporter in rat brain astrocytes', Endocrinology, vol. 128, no. 5, pp. 2548-2557.
Masters, Brian A. ; Werner, Haim ; Roberts, Charles ; Leroith, Derek ; Raizada, Mohan K. / Developmental regulation of insulin-like growth factor-I-stimulated glucose transporter in rat brain astrocytes. In: Endocrinology. 1991 ; Vol. 128, No. 5. pp. 2548-2557.
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