Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes

Cecilia Cheung, Robert A. Brace

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Objectives: Vascularity of the surface of the placenta in humans and of the placenta and fetal membranes in several species including sheep is an important determinant of intramembranous absorption of amniotic fluid. Our previous studies have shown that the total blood vessel surface area in ovine amnion and chorion increases with advancing gestation. Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability factor and is found to be expressed in the ovine placenta and fetal membranes. To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels, the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors, kinase insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), in ovine placenta, chorion, and amnion. Methods: Total RNA was extracted from placental cotyledon, chorion, and amnion of ovine fetuses at 60-140 days of gestation. The relative abundance of VEGF, KDR, and Flt-1 mRNA was determined by Northern blot analysis, and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction. The gestational changes in mRNA levels of VEGF and its receptors were analyzed by regression analysis. Results: In ovine placenta, chorion, and amnion, VEGF mRNA levels increased significantly from 60 to 140 days. The major VEGF molecular form expressed in these tissues was VEGF164, whereas VEGF120, VEGF144, and VEGF188 were present at lower levels. In the placenta, KDR was the primary VEGF receptor expressed, although Flt-1 was also detected at very low levels. In the amnion and chorion, KDR was the only receptor expressed. A gestational-dependent change in VEGF receptor expression was not observed in the placenta and membranes. Conclusions: The increase in VEGF gene expression with advancing gestation in the amnion and chorion where KDR is expressed suggests that VEGF and its receptor are important determinants of vascularity and permeability, and thus exchange capacity, of the intramembranous pathway. Copyright (C) 1999 Society for Gynecologic Investigation.

Original languageEnglish (US)
Pages (from-to)179-185
Number of pages7
JournalJournal of the Society for Gynecologic Investigation
Volume6
Issue number4
DOIs
StatePublished - Jul 1999
Externally publishedYes

Fingerprint

Extraembryonic Membranes
Vascular Endothelial Growth Factor Receptor
Chorion
Amnion
Placenta
Vascular Endothelial Growth Factor A
Vascular Endothelial Growth Factor Receptor-2
Sheep
Pregnancy
Messenger RNA
Permeability
Vascular Endothelial Growth Factor Receptor-1
Gene Expression
Cotyledon
Angiogenesis Inducing Agents
Amniotic Fluid
Microvessels
Reverse Transcriptase Polymerase Chain Reaction
Northern Blotting
Blood Vessels

Keywords

  • Amnion
  • Chorion
  • Development
  • Growth factor
  • Intramembranous pathway
  • Sheep

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes. / Cheung, Cecilia; Brace, Robert A.

In: Journal of the Society for Gynecologic Investigation, Vol. 6, No. 4, 07.1999, p. 179-185.

Research output: Contribution to journalArticle

@article{0102460816b84958b38b2fa698f7f455,
title = "Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes",
abstract = "Objectives: Vascularity of the surface of the placenta in humans and of the placenta and fetal membranes in several species including sheep is an important determinant of intramembranous absorption of amniotic fluid. Our previous studies have shown that the total blood vessel surface area in ovine amnion and chorion increases with advancing gestation. Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability factor and is found to be expressed in the ovine placenta and fetal membranes. To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels, the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors, kinase insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), in ovine placenta, chorion, and amnion. Methods: Total RNA was extracted from placental cotyledon, chorion, and amnion of ovine fetuses at 60-140 days of gestation. The relative abundance of VEGF, KDR, and Flt-1 mRNA was determined by Northern blot analysis, and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction. The gestational changes in mRNA levels of VEGF and its receptors were analyzed by regression analysis. Results: In ovine placenta, chorion, and amnion, VEGF mRNA levels increased significantly from 60 to 140 days. The major VEGF molecular form expressed in these tissues was VEGF164, whereas VEGF120, VEGF144, and VEGF188 were present at lower levels. In the placenta, KDR was the primary VEGF receptor expressed, although Flt-1 was also detected at very low levels. In the amnion and chorion, KDR was the only receptor expressed. A gestational-dependent change in VEGF receptor expression was not observed in the placenta and membranes. Conclusions: The increase in VEGF gene expression with advancing gestation in the amnion and chorion where KDR is expressed suggests that VEGF and its receptor are important determinants of vascularity and permeability, and thus exchange capacity, of the intramembranous pathway. Copyright (C) 1999 Society for Gynecologic Investigation.",
keywords = "Amnion, Chorion, Development, Growth factor, Intramembranous pathway, Sheep",
author = "Cecilia Cheung and Brace, {Robert A.}",
year = "1999",
month = "7",
doi = "10.1016/S1071-5576(99)00016-7",
language = "English (US)",
volume = "6",
pages = "179--185",
journal = "Reproductive Sciences",
issn = "1933-7191",
publisher = "SAGE Publications Inc.",
number = "4",

}

TY - JOUR

T1 - Developmental expression of vascular endothelial growth factor and its receptors in ovine placenta and fetal membranes

AU - Cheung, Cecilia

AU - Brace, Robert A.

PY - 1999/7

Y1 - 1999/7

N2 - Objectives: Vascularity of the surface of the placenta in humans and of the placenta and fetal membranes in several species including sheep is an important determinant of intramembranous absorption of amniotic fluid. Our previous studies have shown that the total blood vessel surface area in ovine amnion and chorion increases with advancing gestation. Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability factor and is found to be expressed in the ovine placenta and fetal membranes. To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels, the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors, kinase insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), in ovine placenta, chorion, and amnion. Methods: Total RNA was extracted from placental cotyledon, chorion, and amnion of ovine fetuses at 60-140 days of gestation. The relative abundance of VEGF, KDR, and Flt-1 mRNA was determined by Northern blot analysis, and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction. The gestational changes in mRNA levels of VEGF and its receptors were analyzed by regression analysis. Results: In ovine placenta, chorion, and amnion, VEGF mRNA levels increased significantly from 60 to 140 days. The major VEGF molecular form expressed in these tissues was VEGF164, whereas VEGF120, VEGF144, and VEGF188 were present at lower levels. In the placenta, KDR was the primary VEGF receptor expressed, although Flt-1 was also detected at very low levels. In the amnion and chorion, KDR was the only receptor expressed. A gestational-dependent change in VEGF receptor expression was not observed in the placenta and membranes. Conclusions: The increase in VEGF gene expression with advancing gestation in the amnion and chorion where KDR is expressed suggests that VEGF and its receptor are important determinants of vascularity and permeability, and thus exchange capacity, of the intramembranous pathway. Copyright (C) 1999 Society for Gynecologic Investigation.

AB - Objectives: Vascularity of the surface of the placenta in humans and of the placenta and fetal membranes in several species including sheep is an important determinant of intramembranous absorption of amniotic fluid. Our previous studies have shown that the total blood vessel surface area in ovine amnion and chorion increases with advancing gestation. Vascular endothelial growth factor (VEGF) is a potent angiogenic and permeability factor and is found to be expressed in the ovine placenta and fetal membranes. To investigate the role of VEGF in maintaining the absorptive function of the intramembranous microvessels, the present study was undertaken to determine the gestational change in gene expression of VEGF and its receptors, kinase insert domain-containing receptor (KDR) and fms-like tyrosine kinase (Flt-1), in ovine placenta, chorion, and amnion. Methods: Total RNA was extracted from placental cotyledon, chorion, and amnion of ovine fetuses at 60-140 days of gestation. The relative abundance of VEGF, KDR, and Flt-1 mRNA was determined by Northern blot analysis, and VEGF molecular forms expressed were identified by reverse transcriptase polymerase chain reaction. The gestational changes in mRNA levels of VEGF and its receptors were analyzed by regression analysis. Results: In ovine placenta, chorion, and amnion, VEGF mRNA levels increased significantly from 60 to 140 days. The major VEGF molecular form expressed in these tissues was VEGF164, whereas VEGF120, VEGF144, and VEGF188 were present at lower levels. In the placenta, KDR was the primary VEGF receptor expressed, although Flt-1 was also detected at very low levels. In the amnion and chorion, KDR was the only receptor expressed. A gestational-dependent change in VEGF receptor expression was not observed in the placenta and membranes. Conclusions: The increase in VEGF gene expression with advancing gestation in the amnion and chorion where KDR is expressed suggests that VEGF and its receptor are important determinants of vascularity and permeability, and thus exchange capacity, of the intramembranous pathway. Copyright (C) 1999 Society for Gynecologic Investigation.

KW - Amnion

KW - Chorion

KW - Development

KW - Growth factor

KW - Intramembranous pathway

KW - Sheep

UR - http://www.scopus.com/inward/record.url?scp=0032838833&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032838833&partnerID=8YFLogxK

U2 - 10.1016/S1071-5576(99)00016-7

DO - 10.1016/S1071-5576(99)00016-7

M3 - Article

VL - 6

SP - 179

EP - 185

JO - Reproductive Sciences

JF - Reproductive Sciences

SN - 1933-7191

IS - 4

ER -