Developmental expression of rat insulin-like growth factor-II/mannose 6-phosphate receptor messenger ribonucleic acid

Mark M. Sklar, Cheryl L. Thomas, Giovanna Municchi, Charles Roberts, Derek LeRoith, Wieland Kiess, Peter Nissley

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We have examined the developmental pattern of the insulin-like growth factor-II (IGF-II)/mannose 6-phosphate (M6P) receptor mRNA in various rat tissues from 20-day gestation fetuses and 20-day postnatal animals by Northern blotting and solution hybridization/RNase protection assays. The major mRNA species in all fetal and postnatal tissues was 9.0 kilobases. The rank order of receptor mRNA concentrations among the fetal tissues was heart > limb/muscle, lung, intestine, kidney, liver > brain, which agrees with the previously reported rank order of the tissue concentrations of receptor protein. The concentration of IGF-II/M6P receptor mRNA was significantly lower in postnatal tissues, again reflecting the relative levels of receptor protein in fetal and postnatal tissues. We measured IGF-II/M6P receptor mRNA copy number in fetal heart, the tissue with the highest concentration of receptor protein and mRNA, by including in the solution hybridization/RNase protection assay known amounts of a sense strand transcript of the receptor cDNA. This sense strand standard was quantitated by incorporating a tracer amount of [32P]UTP into the transcript and measuring the radioactivity in the product purified by gel electrophoresis. The receptor mRNA copy number in fetal heart was 74 molecules/cell. We conclude that the IGF-II/M6P receptor mRNA concentration is an important determinant of the level of receptor protein in most tissues.

Original languageEnglish (US)
Pages (from-to)3484-3491
Number of pages8
JournalEndocrinology
Volume130
Issue number6
StatePublished - Jun 1992
Externally publishedYes

Fingerprint

IGF Type 2 Receptor
RNA
Messenger RNA
Insulin-Like Growth Factor II
Fetus
Fetal Heart
Ribonucleases
Proteins
Uridine Triphosphate
rat insulin-like growth factor II
Northern Blotting
Radioactivity
Intestines
Electrophoresis
Extremities
Complementary DNA
Gels
Kidney
Pregnancy
Muscles

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Sklar, M. M., Thomas, C. L., Municchi, G., Roberts, C., LeRoith, D., Kiess, W., & Nissley, P. (1992). Developmental expression of rat insulin-like growth factor-II/mannose 6-phosphate receptor messenger ribonucleic acid. Endocrinology, 130(6), 3484-3491.

Developmental expression of rat insulin-like growth factor-II/mannose 6-phosphate receptor messenger ribonucleic acid. / Sklar, Mark M.; Thomas, Cheryl L.; Municchi, Giovanna; Roberts, Charles; LeRoith, Derek; Kiess, Wieland; Nissley, Peter.

In: Endocrinology, Vol. 130, No. 6, 06.1992, p. 3484-3491.

Research output: Contribution to journalArticle

Sklar, MM, Thomas, CL, Municchi, G, Roberts, C, LeRoith, D, Kiess, W & Nissley, P 1992, 'Developmental expression of rat insulin-like growth factor-II/mannose 6-phosphate receptor messenger ribonucleic acid', Endocrinology, vol. 130, no. 6, pp. 3484-3491.
Sklar, Mark M. ; Thomas, Cheryl L. ; Municchi, Giovanna ; Roberts, Charles ; LeRoith, Derek ; Kiess, Wieland ; Nissley, Peter. / Developmental expression of rat insulin-like growth factor-II/mannose 6-phosphate receptor messenger ribonucleic acid. In: Endocrinology. 1992 ; Vol. 130, No. 6. pp. 3484-3491.
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