Developmental competence of oocytes after ICSI in the rhesus monkey

K. D. Nusser, Shoukhrat Mitalipov, A. Widmann, B. Gerami-Naini, R. R. Yeoman, D. P. Wolf

    Research output: Contribution to journalArticle

    42 Citations (Scopus)

    Abstract

    Oocyte quantity and quality are critical to assisted reproductive technology (ART), yet few assessments beyond counting metaphase II (MII) oocytes exist. In this study, 30 ± 2 oocytes per cycle were recovered from rhesus monkeys subjected to follicular stimulation with human gonadotrophins, of which 15 ± 1 were MII. Oocyte quality was investigated by monitoring the developmental potential of oocytes subjected to intracytoplasmic sperm injection (ICSI). Despite uniform fertilization rates (71 ± 4%), progression of embryos to blastocysts varied when expressed as a monthly average, from 20 to 85%, with lows from February to April and again in October, which could be attributed to developmental failure of a significant number of oocyte cohorts (14 of 55). Blastocyst rates, after elimination of failed cohorts, were uniform over time (59 ± 4%). Neither culture conditions, the number of follicular stimulations, nor the individual sperm or oocyte donor were associated specifically with developmental failure, suggesting that intrinsic differences between stimulation cycles account for the observed variation in developmental potential. The in-vivo developmental competence of ICSI-produced embryos grown to blastocysts in vitro was also assessed. Two ongoing pregnancies and the birth of a normal female, 'Blastulina', represent landmarks in efforts to expand the use of ART in the rhesus monkey.

    Original languageEnglish (US)
    Pages (from-to)130-137
    Number of pages8
    JournalHuman Reproduction
    Volume16
    Issue number1
    StatePublished - 2001

    Fingerprint

    Intracytoplasmic Sperm Injections
    Macaca mulatta
    Mental Competency
    Oocytes
    Blastocyst
    Assisted Reproductive Techniques
    Metaphase
    Embryonic Structures
    Gonadotropins
    Fertilization
    Spermatozoa
    Parturition
    Pregnancy

    Keywords

    • Blastocyst
    • Embryo transfer
    • ICSI
    • Monkey
    • Oocyte quality

    ASJC Scopus subject areas

    • Physiology
    • Developmental Biology
    • Obstetrics and Gynecology
    • Reproductive Medicine

    Cite this

    Nusser, K. D., Mitalipov, S., Widmann, A., Gerami-Naini, B., Yeoman, R. R., & Wolf, D. P. (2001). Developmental competence of oocytes after ICSI in the rhesus monkey. Human Reproduction, 16(1), 130-137.

    Developmental competence of oocytes after ICSI in the rhesus monkey. / Nusser, K. D.; Mitalipov, Shoukhrat; Widmann, A.; Gerami-Naini, B.; Yeoman, R. R.; Wolf, D. P.

    In: Human Reproduction, Vol. 16, No. 1, 2001, p. 130-137.

    Research output: Contribution to journalArticle

    Nusser, KD, Mitalipov, S, Widmann, A, Gerami-Naini, B, Yeoman, RR & Wolf, DP 2001, 'Developmental competence of oocytes after ICSI in the rhesus monkey', Human Reproduction, vol. 16, no. 1, pp. 130-137.
    Nusser KD, Mitalipov S, Widmann A, Gerami-Naini B, Yeoman RR, Wolf DP. Developmental competence of oocytes after ICSI in the rhesus monkey. Human Reproduction. 2001;16(1):130-137.
    Nusser, K. D. ; Mitalipov, Shoukhrat ; Widmann, A. ; Gerami-Naini, B. ; Yeoman, R. R. ; Wolf, D. P. / Developmental competence of oocytes after ICSI in the rhesus monkey. In: Human Reproduction. 2001 ; Vol. 16, No. 1. pp. 130-137.
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