Developmental changes in synaptic distribution in arcuate nucleus neurons

Arian F. Baquero, Melissa A. Kirigiti, Karalee C. Baquero, Shin J. Lee, M (Susan) Smith, Kevin Grove

    Research output: Contribution to journalArticle

    7 Citations (Scopus)

    Abstract

    Neurons coexpressing neuropeptide Y, agouti-related peptide, and GABA (NAG) play an important role in ingestive behavior and are located in the arcuate nucleus of the hypothalamus. NAG neurons receive both GABAergic and glutamatergic synaptic inputs, however, the developmental time course of synaptic input organization of NAG neurons in mice is unknown. In this study, we show that these neurons have low numbers of GABAergic synapses and that GABA is inhibitory to NAG neurons during early postnatal period. In contrast, glutamatergic inputs onto NAG neurons are relatively abundant by P13 and are comparatively similar to the levels observed in the adult. As mice reach adulthood (9-10 weeks), GABAergic tone ontoNAGneurons increases. At this age,NAGneurons received similar numbers of inhibitory and EPSCs. To further differentiate age-associated changes in synaptic distribution, 17- to 18-week-old lean and diet-induced obesity (DIO) mice were studied. Surprisingly, NAG neurons from lean adult mice exhibit a reduction in the GABAergic synapses compared with younger adults. Conversely, DIO mice display reductions in the number of GABAergic and glutamatergic inputs onto NAG neurons. Based on these experiments, we propose that synaptic distribution in NAG neurons is continuously restructuring throughout development to accommodate the animals’ energy requirements.

    Original languageEnglish (US)
    Pages (from-to)8558-8569
    Number of pages12
    JournalJournal of Neuroscience
    Volume35
    Issue number22
    DOIs
    StatePublished - Jun 3 2015

    Fingerprint

    Arcuate Nucleus of Hypothalamus
    Neurons
    Synapses
    gamma-Aminobutyric Acid
    Obesity
    Diet
    Neuropeptide Y
    Young Adult
    Peptides

    Keywords

    • Arcuate
    • Development
    • NAG neurons
    • Obesity
    • Synaptic inputs

    ASJC Scopus subject areas

    • Neuroscience(all)

    Cite this

    Baquero, A. F., Kirigiti, M. A., Baquero, K. C., Lee, S. J., Smith, M. S., & Grove, K. (2015). Developmental changes in synaptic distribution in arcuate nucleus neurons. Journal of Neuroscience, 35(22), 8558-8569. https://doi.org/10.1523/JNEUROSCI.0058-15.2015

    Developmental changes in synaptic distribution in arcuate nucleus neurons. / Baquero, Arian F.; Kirigiti, Melissa A.; Baquero, Karalee C.; Lee, Shin J.; Smith, M (Susan); Grove, Kevin.

    In: Journal of Neuroscience, Vol. 35, No. 22, 03.06.2015, p. 8558-8569.

    Research output: Contribution to journalArticle

    Baquero, AF, Kirigiti, MA, Baquero, KC, Lee, SJ, Smith, MS & Grove, K 2015, 'Developmental changes in synaptic distribution in arcuate nucleus neurons', Journal of Neuroscience, vol. 35, no. 22, pp. 8558-8569. https://doi.org/10.1523/JNEUROSCI.0058-15.2015
    Baquero AF, Kirigiti MA, Baquero KC, Lee SJ, Smith MS, Grove K. Developmental changes in synaptic distribution in arcuate nucleus neurons. Journal of Neuroscience. 2015 Jun 3;35(22):8558-8569. https://doi.org/10.1523/JNEUROSCI.0058-15.2015
    Baquero, Arian F. ; Kirigiti, Melissa A. ; Baquero, Karalee C. ; Lee, Shin J. ; Smith, M (Susan) ; Grove, Kevin. / Developmental changes in synaptic distribution in arcuate nucleus neurons. In: Journal of Neuroscience. 2015 ; Vol. 35, No. 22. pp. 8558-8569.
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