Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla

Fan Si, Hilary Brodie, Peter Barr-Gillespie, Ana E. Vazquez, Ebenezer N. Yamoah

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Hair cells, the sensory receptors of auditory and vestibular systems, use a transducer apparatus that renders them remarkably sensitive to mechanical displacement as minute as 1 nm. To study the embryonic development of the transducer apparatus in hair cells of the chick auditory papilla, we examined hair cells that have been labeled with N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridiniumdibromide, which has been shown to permeate the transducer channels. In addition, mechanotransduction currents were recorded directly using whole-cell patch-clamp techniques. The structure of the hair bundle was examined using scanning electron microscopy, and immunofluorescence labeling for myosin 1c, myosin 7a, and plasma membrane Ca2+ ATPase 2 was studied to determine the developmental expression of these proteins in embryonic chick papillas. We demonstrate that the transducer apparatus is assembled jointly at embryonic day 11 (E11) of the developing chick basilar papilla. The resting open probability of the transducer channels was high at E12 (∼0.5) and remained substantially elevated at E14-16; it then declined to the mature value of ∼0.15 at E21. The displacement sensitivity of the transduction apparatus, the gating force, increased from E12 to E21. Although the expression of different components of the transducer apparatus and the transduction current peaked at ∼E14-16, marked refinement occurred beyond E16. For example, myosin 1c appeared diffusely localized in hair bundles from E12 to E16, but subsequently consolidated into punctate pattern. The fine temporal and precise spatial assembly of the transducer apparatus likely contributes toward the exquisite sensitivity of the transduction ensemble.

Original languageEnglish (US)
Pages (from-to)10815-10826
Number of pages12
JournalJournal of Neuroscience
Volume23
Issue number34
StatePublished - Nov 26 2003

Fingerprint

Organ of Corti
Transducers
Myosins
Auditory Hair Cells
Calcium-Transporting ATPases
Patch-Clamp Techniques
Sensory Receptor Cells
Electron Scanning Microscopy
Embryonic Development
Fluorescent Antibody Technique
Cell Membrane

Keywords

  • Hearing
  • Inner ear
  • Myosin
  • PMCA
  • Sensory receptors
  • Voltage clamp

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Si, F., Brodie, H., Barr-Gillespie, P., Vazquez, A. E., & Yamoah, E. N. (2003). Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla. Journal of Neuroscience, 23(34), 10815-10826.

Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla. / Si, Fan; Brodie, Hilary; Barr-Gillespie, Peter; Vazquez, Ana E.; Yamoah, Ebenezer N.

In: Journal of Neuroscience, Vol. 23, No. 34, 26.11.2003, p. 10815-10826.

Research output: Contribution to journalArticle

Si, F, Brodie, H, Barr-Gillespie, P, Vazquez, AE & Yamoah, EN 2003, 'Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla', Journal of Neuroscience, vol. 23, no. 34, pp. 10815-10826.
Si, Fan ; Brodie, Hilary ; Barr-Gillespie, Peter ; Vazquez, Ana E. ; Yamoah, Ebenezer N. / Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla. In: Journal of Neuroscience. 2003 ; Vol. 23, No. 34. pp. 10815-10826.
@article{dcc66a5d6ef54a8fbef4008d2517d2d8,
title = "Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla",
abstract = "Hair cells, the sensory receptors of auditory and vestibular systems, use a transducer apparatus that renders them remarkably sensitive to mechanical displacement as minute as 1 nm. To study the embryonic development of the transducer apparatus in hair cells of the chick auditory papilla, we examined hair cells that have been labeled with N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridiniumdibromide, which has been shown to permeate the transducer channels. In addition, mechanotransduction currents were recorded directly using whole-cell patch-clamp techniques. The structure of the hair bundle was examined using scanning electron microscopy, and immunofluorescence labeling for myosin 1c, myosin 7a, and plasma membrane Ca2+ ATPase 2 was studied to determine the developmental expression of these proteins in embryonic chick papillas. We demonstrate that the transducer apparatus is assembled jointly at embryonic day 11 (E11) of the developing chick basilar papilla. The resting open probability of the transducer channels was high at E12 (∼0.5) and remained substantially elevated at E14-16; it then declined to the mature value of ∼0.15 at E21. The displacement sensitivity of the transduction apparatus, the gating force, increased from E12 to E21. Although the expression of different components of the transducer apparatus and the transduction current peaked at ∼E14-16, marked refinement occurred beyond E16. For example, myosin 1c appeared diffusely localized in hair bundles from E12 to E16, but subsequently consolidated into punctate pattern. The fine temporal and precise spatial assembly of the transducer apparatus likely contributes toward the exquisite sensitivity of the transduction ensemble.",
keywords = "Hearing, Inner ear, Myosin, PMCA, Sensory receptors, Voltage clamp",
author = "Fan Si and Hilary Brodie and Peter Barr-Gillespie and Vazquez, {Ana E.} and Yamoah, {Ebenezer N.}",
year = "2003",
month = "11",
day = "26",
language = "English (US)",
volume = "23",
pages = "10815--10826",
journal = "Journal of Neuroscience",
issn = "0270-6474",
publisher = "Society for Neuroscience",
number = "34",

}

TY - JOUR

T1 - Developmental Assembly of Transduction Apparatus in Chick Basilar Papilla

AU - Si, Fan

AU - Brodie, Hilary

AU - Barr-Gillespie, Peter

AU - Vazquez, Ana E.

AU - Yamoah, Ebenezer N.

PY - 2003/11/26

Y1 - 2003/11/26

N2 - Hair cells, the sensory receptors of auditory and vestibular systems, use a transducer apparatus that renders them remarkably sensitive to mechanical displacement as minute as 1 nm. To study the embryonic development of the transducer apparatus in hair cells of the chick auditory papilla, we examined hair cells that have been labeled with N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridiniumdibromide, which has been shown to permeate the transducer channels. In addition, mechanotransduction currents were recorded directly using whole-cell patch-clamp techniques. The structure of the hair bundle was examined using scanning electron microscopy, and immunofluorescence labeling for myosin 1c, myosin 7a, and plasma membrane Ca2+ ATPase 2 was studied to determine the developmental expression of these proteins in embryonic chick papillas. We demonstrate that the transducer apparatus is assembled jointly at embryonic day 11 (E11) of the developing chick basilar papilla. The resting open probability of the transducer channels was high at E12 (∼0.5) and remained substantially elevated at E14-16; it then declined to the mature value of ∼0.15 at E21. The displacement sensitivity of the transduction apparatus, the gating force, increased from E12 to E21. Although the expression of different components of the transducer apparatus and the transduction current peaked at ∼E14-16, marked refinement occurred beyond E16. For example, myosin 1c appeared diffusely localized in hair bundles from E12 to E16, but subsequently consolidated into punctate pattern. The fine temporal and precise spatial assembly of the transducer apparatus likely contributes toward the exquisite sensitivity of the transduction ensemble.

AB - Hair cells, the sensory receptors of auditory and vestibular systems, use a transducer apparatus that renders them remarkably sensitive to mechanical displacement as minute as 1 nm. To study the embryonic development of the transducer apparatus in hair cells of the chick auditory papilla, we examined hair cells that have been labeled with N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridiniumdibromide, which has been shown to permeate the transducer channels. In addition, mechanotransduction currents were recorded directly using whole-cell patch-clamp techniques. The structure of the hair bundle was examined using scanning electron microscopy, and immunofluorescence labeling for myosin 1c, myosin 7a, and plasma membrane Ca2+ ATPase 2 was studied to determine the developmental expression of these proteins in embryonic chick papillas. We demonstrate that the transducer apparatus is assembled jointly at embryonic day 11 (E11) of the developing chick basilar papilla. The resting open probability of the transducer channels was high at E12 (∼0.5) and remained substantially elevated at E14-16; it then declined to the mature value of ∼0.15 at E21. The displacement sensitivity of the transduction apparatus, the gating force, increased from E12 to E21. Although the expression of different components of the transducer apparatus and the transduction current peaked at ∼E14-16, marked refinement occurred beyond E16. For example, myosin 1c appeared diffusely localized in hair bundles from E12 to E16, but subsequently consolidated into punctate pattern. The fine temporal and precise spatial assembly of the transducer apparatus likely contributes toward the exquisite sensitivity of the transduction ensemble.

KW - Hearing

KW - Inner ear

KW - Myosin

KW - PMCA

KW - Sensory receptors

KW - Voltage clamp

UR - http://www.scopus.com/inward/record.url?scp=0344629711&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0344629711&partnerID=8YFLogxK

M3 - Article

C2 - 14645474

AN - SCOPUS:0344629711

VL - 23

SP - 10815

EP - 10826

JO - Journal of Neuroscience

JF - Journal of Neuroscience

SN - 0270-6474

IS - 34

ER -