Developmental analysis of the cytomegalovirus enhancer in transgenic animals

John F. Baskar, Patricia P. Smith, Gary Ciment, Stefan Hoffmann, Chandra Tucker, Daniel J. Tenney, Anamaris M. Colberg-Poley, Jay Nelson, Peter Ghazal

Research output: Contribution to journalArticle

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Abstract

The major immediate-early promoter (MIEP) of human cytomegalovirus (HCMV) constitutes a primary genetic switch for viral activation. In this study, regulation of the enhancer-containing segment (nucleotides -670 to +54) of the HCMV MIEP attached to the lacZ reporter gene was examined in the developing embryos of transgenic mice to identify temporal and tissue- specific expression. We find that the transgene reporter is first detected as a dorsal stripe of expression in the neural folds of embryos at day 8.5 postcoitum (p.c.). A broad expression pattern is exhibited in embryos at day 9.5 p.c. This pattern becomes more restricted by day 10.5 p.c. as organogenesis progresses. By day 14.5 p.c., prominent expression is observed in a subpopulation of central nervous system cells and spinal ganglia, endothelial cells, muscle, skin, thyroid, parathyroid, kidney, lung, liver, and gut cells, and the pancreas and submandibular and pituitary glands. This distribution pattern is discussed in relation to human congenital HCMV infection. These results suggest that the transcriptional activity of the HCMV MIEP may determine in part, the ability of the virus to specifically target developing fetal tissues in utero.

Original languageEnglish (US)
Pages (from-to)3215-3226
Number of pages12
JournalJournal of Virology
Volume70
Issue number5
StatePublished - 1996

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Human herpesvirus 5
Cytomegalovirus
transgenic animals
Genetically Modified Animals
embryo (animal)
Embryonic Structures
promoter regions
Virus Activation
Lac Operon
Organogenesis
Neural Crest
pituitary gland
Submandibular Gland
Cytomegalovirus Infections
Spinal Ganglia
Pituitary Gland
organogenesis
Transgenes
Reporter Genes
reporter genes

ASJC Scopus subject areas

  • Immunology

Cite this

Baskar, J. F., Smith, P. P., Ciment, G., Hoffmann, S., Tucker, C., Tenney, D. J., ... Ghazal, P. (1996). Developmental analysis of the cytomegalovirus enhancer in transgenic animals. Journal of Virology, 70(5), 3215-3226.

Developmental analysis of the cytomegalovirus enhancer in transgenic animals. / Baskar, John F.; Smith, Patricia P.; Ciment, Gary; Hoffmann, Stefan; Tucker, Chandra; Tenney, Daniel J.; Colberg-Poley, Anamaris M.; Nelson, Jay; Ghazal, Peter.

In: Journal of Virology, Vol. 70, No. 5, 1996, p. 3215-3226.

Research output: Contribution to journalArticle

Baskar, JF, Smith, PP, Ciment, G, Hoffmann, S, Tucker, C, Tenney, DJ, Colberg-Poley, AM, Nelson, J & Ghazal, P 1996, 'Developmental analysis of the cytomegalovirus enhancer in transgenic animals', Journal of Virology, vol. 70, no. 5, pp. 3215-3226.
Baskar JF, Smith PP, Ciment G, Hoffmann S, Tucker C, Tenney DJ et al. Developmental analysis of the cytomegalovirus enhancer in transgenic animals. Journal of Virology. 1996;70(5):3215-3226.
Baskar, John F. ; Smith, Patricia P. ; Ciment, Gary ; Hoffmann, Stefan ; Tucker, Chandra ; Tenney, Daniel J. ; Colberg-Poley, Anamaris M. ; Nelson, Jay ; Ghazal, Peter. / Developmental analysis of the cytomegalovirus enhancer in transgenic animals. In: Journal of Virology. 1996 ; Vol. 70, No. 5. pp. 3215-3226.
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