Development of phosphorylation site-specific antibodies to nuclear receptors.

Inés Pineda Torra, Julia Staverosky, Susan Ha, Susan K. Logan, Michael J. Garabedian

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Protein phosphorylation is a versatile posttranslational modification that can regulate nuclear receptor function. Although the precise role of receptor phosphorylation is not fully understood, it appears that it functions to direct or refine receptor activity in response to particular physiological requirements. Identifying and characterizing specific nuclear receptor phosphorylation sites is an important step in elucidating the role(s) receptor phosphorylation plays in function. Although traditional methods of metabolic labeling and in vitro protein phosphorylation have been informative, receptor phosphorylation site-specific antibodies are simple and reliable tools to study receptor phosphorylation. This chapter will discuss how to develop nuclear receptor phosphorylation site-specific antibodies to elucidate function.

Original languageEnglish (US)
Pages (from-to)221-235
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume505
DOIs
StatePublished - 2009
Externally publishedYes

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Cytoplasmic and Nuclear Receptors
Phosphorylation
Antibodies
Post Translational Protein Processing
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Development of phosphorylation site-specific antibodies to nuclear receptors. / Torra, Inés Pineda; Staverosky, Julia; Ha, Susan; Logan, Susan K.; Garabedian, Michael J.

In: Methods in molecular biology (Clifton, N.J.), Vol. 505, 2009, p. 221-235.

Research output: Contribution to journalArticle

Torra, Inés Pineda ; Staverosky, Julia ; Ha, Susan ; Logan, Susan K. ; Garabedian, Michael J. / Development of phosphorylation site-specific antibodies to nuclear receptors. In: Methods in molecular biology (Clifton, N.J.). 2009 ; Vol. 505. pp. 221-235.
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