Development of an irradiated rodent model to study flap revascularization

Patrick C. Angelos, Kate E. McCarn, Shelley R. Winn, Tamer Ghanem, Darryl S. Kaurin, John Holland, Mark Wax

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective: To develop a reproducible free-flap animal model to study the effects of irradiation on flap revascularization. Design: After institutional animal care and use committee review and approval, 16 Sprague-Dawley rats were subjected to either 23-or 40-Gy electron beam irradiation to their ventral abdominal wall. After a recovery period, the animals then underwent a ventral fasciocutaneous flap pedicled on the inferior epigastric vessels with subsequent pedicle ligation at 10 days. An additional 16 rats were subjected to 40 Gy of irradiation and underwent pedicle ligation at 8 or 14 days postoperatively to determine if time to pedicle ligation affected percentage of flap viability. Results: Rats receiving 23 Gy of irradiation had the same viability as rats undergoing no radiation. Rats receiving 40 Gy of irradiation had a significantly lower average percentage of flap viability (56.9%) than animals receiving 23 Gy (90.9%) (P

Original languageEnglish (US)
Pages (from-to)119-122
Number of pages4
JournalArchives of Facial Plastic Surgery
Volume12
Issue number2
DOIs
StatePublished - Mar 2010

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Rodentia
Ligation
Animal Care Committees
Surgical Flaps
Free Tissue Flaps
Abdominal Wall
Sprague Dawley Rats
Animal Models
Electrons
Radiation

ASJC Scopus subject areas

  • Surgery
  • Medicine(all)

Cite this

Development of an irradiated rodent model to study flap revascularization. / Angelos, Patrick C.; McCarn, Kate E.; Winn, Shelley R.; Ghanem, Tamer; Kaurin, Darryl S.; Holland, John; Wax, Mark.

In: Archives of Facial Plastic Surgery, Vol. 12, No. 2, 03.2010, p. 119-122.

Research output: Contribution to journalArticle

Angelos, Patrick C. ; McCarn, Kate E. ; Winn, Shelley R. ; Ghanem, Tamer ; Kaurin, Darryl S. ; Holland, John ; Wax, Mark. / Development of an irradiated rodent model to study flap revascularization. In: Archives of Facial Plastic Surgery. 2010 ; Vol. 12, No. 2. pp. 119-122.
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