Development of a tunable wide-range gene induction system useful for the study of streptococcal toxin-antitoxin systems

Zhoujie Xie, Fengxia Qi, Justin Merritt

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Despite the plethora of genetic tools that have been developed for use in Streptococcus mutans, the S. mutans genetic system still lacks an effective gene induction system exhibiting low basal expression and strong inducibility. Consequently, we created two hybrid gene induction cassettes referred to as Xyl-S1 and Xyl-S2. Both Xyl-S cassettes are xylose inducible and controlled by the Bacillus megaterium xylose repressor. The Xyl-S cassettes each demonstrated

Original languageEnglish (US)
Pages (from-to)6375-6384
Number of pages10
JournalApplied and Environmental Microbiology
Volume79
Issue number20
DOIs
StatePublished - 2013
Externally publishedYes

Fingerprint

antitoxins
Antitoxins
Streptococcus mutans
Xylose
gene induction
xylose
toxin
toxins
Bacillus megaterium
gene
Genes

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Food Science
  • Biotechnology
  • Ecology

Cite this

Development of a tunable wide-range gene induction system useful for the study of streptococcal toxin-antitoxin systems. / Xie, Zhoujie; Qi, Fengxia; Merritt, Justin.

In: Applied and Environmental Microbiology, Vol. 79, No. 20, 2013, p. 6375-6384.

Research output: Contribution to journalArticle

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