Development of a method to quantify platelet adhesion and aggregation under static conditions

Sandra M. Baker-Groberg, Flor A. Cianchetti, Kevin G. Phillips, Owen McCarty

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Platelets are important players in hemostasis and thrombosis. Thus, accurate assessment of platelet function is crucial for identifying platelet function disorders and measuring the efficacy of antiplatelet therapies. We have developed a novel platelet aggregation technique that utilizes the physical parameter of platelet concentration in conjunction with volume and mass measurements to evaluate platelet adhesion and aggregation. Platelet aggregates were formed by incubating purified platelets on fibrinogen- or fibrillar collagen-coated surfaces at platelet concentrations ranging from 20,000 to 500,000 platelets/μL. Platelets formed aggregates under static conditions in a platelet concentration-dependent manner, with significantly greater mean volume and mass at higher platelet concentrations (≥400,000 platelets/μL). We show that a platelet glycoprotein IIb/IIIa inhibitor abrogated platelet-platelet aggregation, which significantly reduced the volume and mass of the platelets on the collagen surface. This static platelet aggregation technique is amenable to standardization and represents a useful tool to investigate the mechanism of platelet activation and aggregation under static conditions.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalCellular and Molecular Bioengineering
Volume7
Issue number2
DOIs
StatePublished - 2014

Fingerprint

Platelets
Adhesion
Platelet Aggregation
Aggregation
Quantify
Blood Platelets
Agglomeration
Collagen
Fibrillar Collagens
Integrin beta3
Platelet Glycoprotein GPIIb-IIIa Complex
Platelet Aggregation Inhibitors
Platelet Activation
Hemostasis
Platelet Membrane Glycoproteins
Fibrinogen
Thrombosis
Glycoproteins

Keywords

  • Aggregation
  • Blood
  • Microscopy
  • Platelets

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Development of a method to quantify platelet adhesion and aggregation under static conditions. / Baker-Groberg, Sandra M.; Cianchetti, Flor A.; Phillips, Kevin G.; McCarty, Owen.

In: Cellular and Molecular Bioengineering, Vol. 7, No. 2, 2014, p. 285-290.

Research output: Contribution to journalArticle

Baker-Groberg, Sandra M. ; Cianchetti, Flor A. ; Phillips, Kevin G. ; McCarty, Owen. / Development of a method to quantify platelet adhesion and aggregation under static conditions. In: Cellular and Molecular Bioengineering. 2014 ; Vol. 7, No. 2. pp. 285-290.
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